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- Lipoptena fortisetosa (Diptera: Hippoboscidae) in a Red Deer in the Center of PortugalPublication . Rodrigues, Isabel; Martins, Filipa; Mateus, Teresa LetraDeer keds (Insecta: Diptera: Hippoboscidae: Lipoptena spp.) are haematophagous ectoparasites of wild as well as domestic animals, being most often associated with cervids, but also can infest Bovidae (cattle, goats, chamois, mouflon, antelopes, etc.), and occasionally humans. Upon finding a suitable mammal host, deer keds shed their wings, remaining in a wingless form for the rest of their life. These flies are viviparous species and they generate a larvae that falls to the ground and pupates. In severe infestations, these ectoparasites can be responsible for dermatitis, allergic rhinoconjunctivitis, and in extreme cases, anaphylactic shock when they bite humans. They also potential vectors of several pathogens such as Babesia spp.,Theileria spp., Bartonella spp., Coxiella spp. and Anaplasma spp. Of over 30 species of Lipoptena insects worldwide, two of the six known species in Europe, Lipoptena cervi along with L. fortisetosa, are known to have a relatively wide distribution. We report for the first time, the presence of L. fortisetosa in a red deer (Cervus elaphus) in Castelo Branco district, in the Center of Portugal. Lipoptena fortisetosa was probably introduced in Europe with sika deer from the Eastern Palaearctic and is continuously expanding its range, especially in the Southern part of Europe. There are pertinent morphological differences between the two species, that must be taken in account to facilitate their accurate identification, among others, the external features of the head and antennae, features on the thorax, number and distribution of bristles, and in the external genitalia. This new finding may represent a new challenge to the epidemiology of this species in Portugal, and to the health of its wide range hosts, particularly because it can transmit several disease-causing pathogens to animals, as well as to humans. Further investigations are worthy of attention on Lipoptena species, in Portugal, with a One Health perspective.
- Impacto da suplementação na alimentação de coelhos desmamados com folha de oliveira (OLEA EUROPEIA L.)e/ou selénio no crescimento e parâmetros histológicos cecaisPublication . Gomes, Daniela; Faria, Adriana Cristina de; Takahashi, Sabrina; Correia, Teresa MontenegroA produção de carne de coelho na União Europeia,especialmente em Portugal, enfrenta grandes desafios, devido, entre outras causas ao aumento dos custos deprodução. Em resposta, procuram-se alternativas mais sustentáveis visando minimizar o impacto ambiental e reduzam os custos de produção.Este trabalho investigou o potencial da suplementação de folhas de oliveira, um subproduto muito abundante em Portugal na indústria do azeite e ao qual lhe são atribuídas várias propriedades benéficas para o crescimento e saúde dos coelhos, assim como a suplementação com selénio.
- Exploring the inhibitory potential of 2-styrylchromones on pancreatic α- amylasePublication . Santos, Clementina M.M.; Proença, Carina; Freitas, Marisa; Araújo, Alberto N.; Silva, Artur M.S.; Fernandes, EduardaDiabetes mellitus is a metabolic disorder that afflicts about 537 million people worldwide and this number is predicted to rise to 643 million by 2030, according to the International Diabetes Federation.1 It is characterized by hyperglycemia, caused by the deficiency in the secretion of insulin and/or in the action of this pancreatic hormone. To date, the best therapeutic strategy known consists of inhibiting carbohydrate-hydrolyzing enzymes, namely the α-amylase enzyme. The currently marketed inhibitors (e.g., acarbose, miglitol, and voglibose) are based on carbohydrate-related structures, with moderate affinity for the enzyme and with disturbing side effects.2 Thus, an active pursuit for novel and more effective anti-diabetic drugs has been carried out and a wide variety of structurally diverse heterocyclic compounds has been studied. Chromones are among the oxygenated 6-membered heterocycles evaluated and the results exhibited by some 2-arylchromones point out the relevance of this class of compounds in the inhibition of α‐amylase enzymatic activity.3 Nonetheless, a detailed investigation of the effects of the restricted group of chromones known as 2-styrylchromones (2-SC) has not been conducted to date. With this rationale in mind and as part of our on-going project, the aim of the present study is to investigate the effect of a panel of twelve 2-SC 1 on pancreatic α-amylase activity and their mechanism of inhibition, to infer about the importance of this class of compounds in the management of type 2 diabetes and its complications. α-Amylase was exposed to different concentrations of 2-SC 1 and the hydrolysis of the substrate 2-chloro-p-nitrophenyl-α-D-maltotriose was monitored spectrophotometrically at 405 nm. Acarbose was used as the standard inhibitor. In addition, the study of the inhibition type was carried out through nonlinear regression Michaelis-Menten enzymatic kinetics and the corresponding Lineweaver-Burk plot.4 The results showed that the IC50 values obtained ranged from 26 to 174 μM, considerably higher than the positive control acarbose (IC50 = 0.62 ± 0.07 μM). All active compounds revealed a competitive type of inhibition while for the positive control a mixed type of inhibition was obtained. More details concerning the structure-activity relationship will be presented and discussed in this communication.
- Carbon sequestration in chestnut coppices: impact of shoot density simulated with the CO2Fix modelPublication . Patrício, Maria Sameiro; El Baine, Chaimae; Nunes, LuísThe carbon cycle plays a vital role in maintaining ecosystem stability by regulating the flow of organic matter and influencing global climate dynamics. However, rising CO₂ emissions from human activities have disrupted this balance, driving climate change and increasing the frequency of extreme weather events. Addressing this challenge requires strengthening natural carbon sinks, particularly forests, which store significant amounts of carbon in both biomass and soils. Sweet chestnut (Castanea sativa Mill.) coppice systems are promising for carbon sequestration due to their fast growth and potential for long-term carbon storage. Despite their ecological importance, many chestnut coppices remain unmanaged due to limited economic returns. Innovative strategies, such as payments for ecosystem services, could unlock their potential and enhance their contribution to climate change mitigation while promoting sustainable forest management.This study assesses the carbon sequestration and storage potential of sweet chestnut coppices using the CO₂FIX model, calibrated with field data from northeastern Portugal. The main objectives are to: Calibrate the CO₂FIX model to predict biomass and carbon stocks under different management practices. Validate model accuracy by comparing simulations with observed field data. Optimize parameterization to improved reliability for chestnut coppice systems. Identify growth constraints to support sustainable management strategies.
- Purificação a seco do biodiesel etanólico através de adsorção utilizando materiais à base de casca de amêndoaPublication . Mezzalira, Melissa Giacomet; Gomes, Maria Carolina Sérgi; Queiroz, Ana; Brito, Paulo; Ribeiro, António E.O crescente interesse por fontes de energia sustentáveis tem estimulado a substituição dos combustíveis fósseis1. Nesse contexto, o biodiesel surge como alternativa viável ao diesel convencional, podendo ser produzido a partir de diversas matérias-primas, como o óleo alimentar usado (OAU), promovendo o reaproveitamento eficiente de resíduos. A purificação convencional do biodiesel via lavagem com água, embora eficiente, gera elevados volumes de efluentes. Como alternativa ambientalmente mais sustentável, têm sido investigados materiais adsorventes para a remoção de contaminantes, como o glicerol, com o objetivo de atender à norma EN 14214, que estabelece um limite máximo de glicerol de 0,02% (m/m) no biodiesel.
- Moléculas híbridas de base biológica com propriedades corantes e conservantesPublication . Novais, Cláudia; Molina, Adriana K.; Abreu, Rui M.V.; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R.; Pereira, CarlaA necessidade crescente de alimentar a população mundial em expansão, aliada ao aumento da consciência e das expectativas dos consumidores, tem impulsionado a evolução dos sistemas, processos e produtos na indústria alimentar. Apesar dos avanços significativos feitos no que respeita aos aditivos alimentares, a pesquisa de novas gerações mais seguras e saudáveis tem vindo a ganhar destaque. Os aditivos de origem natural, além de colorir ou conservar, podem oferecer benefícios à saúde. No entanto, enfrentam frequentemente desafios de estabilidade, sustentabilidade e rentabilidade, reforçando a necessidade de soluções inovadoras.
- Estratégia para mascarar o sabor amargo de fármacos em formulações orais pediátricasPublication . Pinto, Susana; Gomes, Joana; Ogbonna, John Dike N.; Cunha, Edite; Attama, Anthony A.; Ofokansi, Kenneth C.; Ferreira, Helena; Marx, Ítala; Peres, António M.; Lobo, José Manuel Sousa; Almeida, Isabel F.A facilidade de administração e a possibilidade de ajuste de dose das formas farmacêuticas líquidas para administração oral, reforçam a sua grande utilidade na população pediátrica
- Identification of the geographical origin of Spanish beech nuts, Fagus sylvatica L., using a chemometric supervised approachPublication . Lamas, Sandra; Rodrigues, Nuno; Herrero, Baudilio; Casal, Susana; Cruz, Rebeca; Pereira, José Alberto; Peres, António M.In general, dry nuts, such as hazelnuts, almonds. chestnuts and walnuts, composition has been investigated and are well characterized. Nevertheless, other less unknown nuts, such as beech nuts have little information. Beech nuts proceed from the Fagus sylvatica L. tree, which is prevalent in central and southem Europe, and is commonly used for oil extraction. A study carried out by Obranovic et al. found that the chemical composition of beech nut oit varies significantly depending on the geographical origin. Siger et al. characterized the oit obtained from beech nut seeds, highlighting its high content in linoleic acid. However, scientific data regarding the physicochemical properties and composiHon of beech nuts are scarce. In this context. this work evaluated whether there are differences in terms of the physicochemical data and composition of the beech nut between regions, by applying a chemometric procedure.
- Application of a lab-made electronic nose as a tool to assess extra virgin olive oil sensory categoryPublication . Ferreiro, Nuno Manuel; Veloso, Ana C.A.; Rodrigues, Nuno; Pereira, José Alberto; Peres, António M.Olive oils assessed by a sensory panel in a national contest and split according to the perceived intensity of the fruitness, green delicate, green medium and green.
- Identification of almond’s variety based on FTIR spectra of ground samplesPublication . Lamas, Sandra; Rodrigues, Nuno; Santamaria-Echart, Arantzazu; Palu, Igor; Manchique, Jocyla R.; Lopéz-Cortés, Isabel; Pereira, José Alberto; Peres, António M.Almond's traceability is of utmost relevance.