Advisor(s)
Abstract(s)
Heat-Assisted Extraction (HAE) was used for the optimized production of an extract rich
in anthocyanin compounds from Ocimum basilicum var. purpurascens leaves. The optimization was
performed using the response surface methodology employing a central composite experimental
design with five-levels for each of the assessed variables. The independent variables studied were
the extraction time (t, 20–120 min), temperature (T, 25–85 C), and solvent (S, 0–100% of ethanol,
v/v). Anthocyanin compounds were analysed by HPLC-DAD-ESI/MS and the extraction yields were
used as response variables. Theoretical models were developed for the obtained experimental data,
then the models were validated by a selected number of statistical tests, and finally, those models
were used in the prediction and optimization steps. The optimal HAE conditions for the extraction
of anthocyanin compounds were: t = 65.37 3.62 min, T = 85.00 1.17 C and S = 62.50 4.24%,
and originated 114.74 0.58 TA mg/g of extract. This study highlighted the red rubin basil leaves
as a promising natural matrix to extract pigmented compounds, using green solvents and reduced
extraction times. The extract rich in anthocyanins also showed antimicrobial and anti-proliferative
properties against four human tumor cell lines, without any toxicity on a primary porcine liver
cell line.
Description
Keywords
Anthocyanins Extraction optimization Heat-Assisted Extraction Natural colorants Ocimum basilicum var. purpurascens leaves Red rubin basil
Pedagogical Context
Citation
Fernandes, Filipa; Pereira, Eliana; Prieto, Miguel A.; Calhelha, Ricardo C.; Ciric, Ana; Soković, Marina; Simal-Gandara, Jesus; Barros, Lillian; Ferreira, Isabel C.F.R. (2019). Optimization of the Extraction Process to Obtain a Colorant Ingredient from Leaves of Ocimum basilicum var. Purpurascens. Molecules. ISSN 1420-3049. 24, p. 1-18