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Optimization of the Extraction Process to Obtain a Colorant Ingredient from Leaves of Ocimum basilicum var. Purpurascens

2019Journal article Open access
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dc.contributor.authorFernandes, Filipa A.
dc.contributor.authorPereira, Eliana
dc.contributor.authorPrieto Lage, Miguel A.
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorĆirić, Ana
dc.contributor.authorSoković, Marina
dc.contributor.authorSimal-Gandara, Jesus
dc.contributor.authorBarros, Lillian
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2020-01-03T17:06:40Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2020-01-03T17:06:40Z
dc.date.issued2019
dc.description.abstractHeat-Assisted Extraction (HAE) was used for the optimized production of an extract rich in anthocyanin compounds from Ocimum basilicum var. purpurascens leaves. The optimization was performed using the response surface methodology employing a central composite experimental design with five-levels for each of the assessed variables. The independent variables studied were the extraction time (t, 20–120 min), temperature (T, 25–85 C), and solvent (S, 0–100% of ethanol, v/v). Anthocyanin compounds were analysed by HPLC-DAD-ESI/MS and the extraction yields were used as response variables. Theoretical models were developed for the obtained experimental data, then the models were validated by a selected number of statistical tests, and finally, those models were used in the prediction and optimization steps. The optimal HAE conditions for the extraction of anthocyanin compounds were: t = 65.37 3.62 min, T = 85.00 1.17 C and S = 62.50 4.24%, and originated 114.74 0.58 TA mg/g of extract. This study highlighted the red rubin basil leaves as a promising natural matrix to extract pigmented compounds, using green solvents and reduced extraction times. The extract rich in anthocyanins also showed antimicrobial and anti-proliferative properties against four human tumor cell lines, without any toxicity on a primary porcine liver cell line.
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Program PT2020 for financial support to CIMO (UID/AGR/00690/2013), Lillian Barros and Ricardo C. Calhelha contracts. The authors are also grateful to the Interreg España-Portugal for financial support through the project 0377_Iberphenol_6_E). This work is funded by the European Regional Development Fund (ERDF) through the Regional Operational Program North 2020, within the scope of Project Mobilizador Norte-01-0247-FEDER-024479: ValorNatural®. Authors are also grateful to Ministry of Education, Science and Technological Development, Republic of Serbia, grant No. 173032. The authors thank the GAIN (Xunta de Galicia) for financial support (P.P. 0000 421S 140.08) to Miguel A. Prieto by a post-doctoral (modality B) grant.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationFernandes, Filipa; Pereira, Eliana; Prieto, Miguel A.; Calhelha, Ricardo C.; Ciric, Ana; Soković, Marina; Simal-Gandara, Jesus; Barros, Lillian; Ferreira, Isabel C.F.R. (2019). Optimization of the Extraction Process to Obtain a Colorant Ingredient from Leaves of Ocimum basilicum var. Purpurascens. Molecules. ISSN 1420-3049. 24, p. 1-18en_EN
dc.identifier.doi10.3390/molecules24040686en_EN
dc.identifier.urihttp://hdl.handle.net/10198/20069
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.subjectAnthocyaninsen_EN
dc.subjectExtraction optimizationen_EN
dc.subjectHeat-Assisted Extractionen_EN
dc.subjectNatural colorantsen_EN
dc.subjectOcimum basilicum var. purpurascens leavesen_EN
dc.subjectRed rubin basilen_EN
dc.titleOptimization of the Extraction Process to Obtain a Colorant Ingredient from Leaves of Ocimum basilicum var. Purpurascensen_EN
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00690%2F2013/PT
oaire.fundingStream5876
person.familyNameFernandes
person.familyNamePereira
person.familyNamePrieto Lage
person.familyNameCalhelha
person.familyNameBarros
person.familyNameFerreira
person.givenNameFilipa Alexandra
person.givenNameEliana
person.givenNameMiguel A.
person.givenNameRicardo C.
person.givenNameLillian
person.givenNameIsabel C.F.R.
person.identifier107688
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person.identifier.orcid0000-0001-6807-1137
person.identifier.orcid0000-0003-2854-6745
person.identifier.orcid0000-0002-3513-0054
person.identifier.orcid0000-0002-6801-4578
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridA-8045-2019
person.identifier.ridQ-8017-2018
person.identifier.ridG-4516-2011
person.identifier.ridJ-2172-2014
person.identifier.ridJ-3600-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id55938912300
person.identifier.scopus-author-id35937495700
person.identifier.scopus-author-id6507978333
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
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