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Advisor(s)
Abstract(s)
Naturally-occurring phytochemicals have received a pivotal attention in the last years,
due to the increasing evidences of biological activities. Equisetum giganteum L.,
commonly known as “giant horsetail”, is a native plant from Central and South
America, being largely used in dietary supplements as diuretic, hemostatic, antiinflammatory
and anti-rheumatic agents [1,2]. The aim of the present study was to
evaluate the antioxidant (scavenging effects on 2,2-diphenyl-1-picrylhydrazyl radicals-
RSA, reducing power- RP, β-carotene bleaching inhibition- CBI and lipid peroxidation
inhibition- LPI), anti-inflammatory (inhibition of NO production in lipopolysaccharidestimulated
RAW 264.7 macrophages) and cytotoxic (in a panel of four human tumor
cell lines: MCF-7- breast adenocarcinoma, NCI-H460- non-small cell lung cancer,
HeLa- cervical carcinoma and HepG2- hepatocellular carcinoma; and in non-tumor
porcine liver primary cells- PLP2) properties of E. giganteum, providing a
phytochemical characterization of its extract (ethanol/water, 80:20, v/v), by using highperformance
liquid chromatography coupled to diode array detection and electrospray
ionisation mass spectrometry (HPLC-DAD–ESI/MS).
E. giganteum presented fourteen phenolic compounds, two phenolic acids and twelve
flavonol glycoside derivatives, mainly kaempferol derivatives, accounting to 81% of the
total phenolic content, being kaempferol-O-glucoside-O-rutinoside, the most abundant
molecule (7.6 mg/g extract). The extract exhibited antioxidant (EC50 values = 123, 136,
202 and 57.4 μg/mL for RSA, RP, CBI and LPI, respectively), anti-inflammatory (EC50
value = 239 μg/mL) and cytotoxic (GI50 values = 250, 258, 268 and 239 μg/mL for
MCF-7, NCI-H460, HeLa and HepG2, respectively) properties, which were positively
correlated with its concentration in phenolic compounds. Furthermore, up to 400
μg/mL, it did not revealed toxicity in non-tumor liver cells.
Thus, this study highlights the potential of E. giganteum extracts as rich sources of
phenolic compounds that can be used in the food, pharmaceutical and cosmetic fields.
Description
Keywords
Equisetum giganteum Antioxidant activity Anti-inflammatory activity Cytotoxic properties Henolic compounds
Citation
Jabeur, Inés; Martins, Natália; Barros, Lillian; Calhelha, Ricardo C.; Vaz, Josiana; Achour, Lotfi; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R. (2016). HPLC-DAD-ESI/MS phenolic characterization and biological activity of Equisetum giganteum L. In Les Septièmes Journées Scientifiques Internationales sur la Valorisation des Bioressources. Monastir, Tunisie