Publication
HPLC-DAD-ESI/MS phenolic characterization and biological activity of Equisetum giganteum L.
| dc.contributor.author | Jabeur, Inès | |
| dc.contributor.author | Martins, Natália | |
| dc.contributor.author | Barros, Lillian | |
| dc.contributor.author | Calhelha, Ricardo C. | |
| dc.contributor.author | Vaz, Josiana A. | |
| dc.contributor.author | Achour, Lotfi | |
| dc.contributor.author | Santos-Buelga, Celestino | |
| dc.contributor.author | Ferreira, Isabel C.F.R. | |
| dc.date.accessioned | 2016-09-19T10:03:50Z | |
| dc.date.available | 2016-09-19T10:03:50Z | |
| dc.date.issued | 2016 | |
| dc.description.abstract | Naturally-occurring phytochemicals have received a pivotal attention in the last years, due to the increasing evidences of biological activities. Equisetum giganteum L., commonly known as “giant horsetail”, is a native plant from Central and South America, being largely used in dietary supplements as diuretic, hemostatic, antiinflammatory and anti-rheumatic agents [1,2]. The aim of the present study was to evaluate the antioxidant (scavenging effects on 2,2-diphenyl-1-picrylhydrazyl radicals- RSA, reducing power- RP, β-carotene bleaching inhibition- CBI and lipid peroxidation inhibition- LPI), anti-inflammatory (inhibition of NO production in lipopolysaccharidestimulated RAW 264.7 macrophages) and cytotoxic (in a panel of four human tumor cell lines: MCF-7- breast adenocarcinoma, NCI-H460- non-small cell lung cancer, HeLa- cervical carcinoma and HepG2- hepatocellular carcinoma; and in non-tumor porcine liver primary cells- PLP2) properties of E. giganteum, providing a phytochemical characterization of its extract (ethanol/water, 80:20, v/v), by using highperformance liquid chromatography coupled to diode array detection and electrospray ionisation mass spectrometry (HPLC-DAD–ESI/MS). E. giganteum presented fourteen phenolic compounds, two phenolic acids and twelve flavonol glycoside derivatives, mainly kaempferol derivatives, accounting to 81% of the total phenolic content, being kaempferol-O-glucoside-O-rutinoside, the most abundant molecule (7.6 mg/g extract). The extract exhibited antioxidant (EC50 values = 123, 136, 202 and 57.4 μg/mL for RSA, RP, CBI and LPI, respectively), anti-inflammatory (EC50 value = 239 μg/mL) and cytotoxic (GI50 values = 250, 258, 268 and 239 μg/mL for MCF-7, NCI-H460, HeLa and HepG2, respectively) properties, which were positively correlated with its concentration in phenolic compounds. Furthermore, up to 400 μg/mL, it did not revealed toxicity in non-tumor liver cells. Thus, this study highlights the potential of E. giganteum extracts as rich sources of phenolic compounds that can be used in the food, pharmaceutical and cosmetic fields. | pt_PT |
| dc.identifier.citation | Jabeur, Inés; Martins, Natália; Barros, Lillian; Calhelha, Ricardo C.; Vaz, Josiana; Achour, Lotfi; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R. (2016). HPLC-DAD-ESI/MS phenolic characterization and biological activity of Equisetum giganteum L. In Les Septièmes Journées Scientifiques Internationales sur la Valorisation des Bioressources. Monastir, Tunisie | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10198/13251 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.relation | PLANTAS MEDICINAIS A UTILIZAR EM FITOTERAPIA COMO ANTI-FÚNGICOS: EFEITO DE COMPOSTOS FENÓLICOS NO CRESCIMENTO DE ESPÉCIES DE CANDIDA EM SUSPENSÃO E EM BIOFILMES | |
| dc.relation | Providing industry and consumers with new alternative sources of natural additives from plant origin in the classes of preservatives and colouring agents: extraction, purification, stabilization and product development | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Equisetum giganteum | pt_PT |
| dc.subject | Antioxidant activity | pt_PT |
| dc.subject | Anti-inflammatory activity | pt_PT |
| dc.subject | Cytotoxic properties | pt_PT |
| dc.subject | Henolic compounds | pt_PT |
| dc.title | HPLC-DAD-ESI/MS phenolic characterization and biological activity of Equisetum giganteum L. | pt_PT |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | PLANTAS MEDICINAIS A UTILIZAR EM FITOTERAPIA COMO ANTI-FÚNGICOS: EFEITO DE COMPOSTOS FENÓLICOS NO CRESCIMENTO DE ESPÉCIES DE CANDIDA EM SUSPENSÃO E EM BIOFILMES | |
| oaire.awardTitle | Providing industry and consumers with new alternative sources of natural additives from plant origin in the classes of preservatives and colouring agents: extraction, purification, stabilization and product development | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F68344%2F2010/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F87658%2F2012/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F107855%2F2015/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FAGR%2FUI0690%2F2014/PT | |
| oaire.citation.conferencePlace | Tunisia | pt_PT |
| oaire.citation.title | Les Septièmes Journées Scientifiques Internationales sur la Valorisation des Bioressources | pt_PT |
| oaire.fundingStream | SFRH | |
| oaire.fundingStream | 5876 | |
| person.familyName | Jabeur | |
| person.familyName | Barros | |
| person.familyName | Calhelha | |
| person.familyName | Vaz | |
| person.familyName | Ferreira | |
| person.givenName | Inès | |
| person.givenName | Lillian | |
| person.givenName | Ricardo C. | |
| person.givenName | Josiana A. | |
| person.givenName | Isabel C.F.R. | |
| person.identifier | 469085 | |
| person.identifier | 144781 | |
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| person.identifier.orcid | 0000-0002-6989-1169 | |
| person.identifier.orcid | 0000-0003-4910-4882 | |
| person.identifier.rid | J-3600-2013 | |
| person.identifier.rid | J-2172-2014 | |
| person.identifier.rid | E-8500-2013 | |
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| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | conferenceObject | pt_PT |
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