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Abstract(s)
Phenolic metabolites are common constituents of fruits and vegetables, and the interest of plant
phenolic extracts derives from the evidence of their potent antioxidant activity and their wide range
of pharmacologic properties including anticancer, antimicrobial and platelet aggregation inhibition
activities. The fruits of Arbutus unedo L. are eaten raw or made in liqueurs (1 ]. In traditional folk
medicine, they are reported as having antiseptic, diuretic and laxative effects, being also used to
treat arterial hypertension (2) .Moreover, the bark or roots decoctions are used as anti-inflammatory,
laxative, carminative, digestive, odontalgic and cardiotonic. In this work, two
different enriched phenolic extracts were prepared from A. unedo frutis, in order to evaluate and
compare their bioactivity: a phenolic extract without anthocyanins (PE; with phenolic acids,
flavones/ols, flavan-3-ols and galloyl derivatives) and a separate anthocyanins extract (AE). A.
unedo PE presented the highest antioxidant activity in all the in vitro assays (free radicals
scavenging activity, reducing power and lipid peroxidation inhibition), which could be related to the
presence of galloyl derivatives. The antiproliferation activity was tested in different human tumour
cell lines (breast, lung, colon, cervical and hepatocellular carcinomas), and the hepatotoxicity was
evaluated using a porcine liver primary cell culture (PLP2). A. unedo PE also gave the highest
antiproliferation inhibition presenting a Gl50 value=38 119/ml for NCI-H460- non-small lung
carcinoma cell line, without toxicity for PLP2 (GI 50>400 119/m l). Overall, the results showed that A.
unedo fruits may have great potential for food industries as a source of colors and flavors, as well
as bioactive molecules such as phenolic compounds for dietary supplements or functional foods.
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Citation
Guimarães, Rafaela; Barros, Lillian; Calhelha, Ricardo C.; Dueñas, Montserrat; Carvalho, Ana Maria; Queiroz, Maria-João R.P.; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R. (2013). Bioactivity and chemical characterization of phenolic and anthocyanin extracts of Arbutus unedo L. wild fruits. In 1st Symposium on Medicinal Chemistry. Braga