| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 342.88 KB | Adobe PDF |
Advisor(s)
Abstract(s)
As the global cosmetic market continues to grow steaduily, there is a continuous demand for product diversification resulting m the emergence of products with different claims and functional properties [l]. Because of the rising consciousness and awareness among consumers about the origin, safety and environmental implications of some of the ingredients used in cosmetic formulations, there is an increasing demand for the use of green and more sustainable raw materials during product formulation. Mushrooms are globally consumed not only because of their nutritional properties but also for their medicinal potential and more recently their utilisation as cosmeceutical ingredients due to their richness in several bioactive compounds [l]. In the present study, the Ganoderma lucidum ethanolic extract was evaluated for antioxidant, anti-inflammatory, anti-tyrosmase, antimicrobial and cytotoxic effects, and further characterized m terms of phenolic acids and triterpenoids using HPLC-DAD-ESI/MSn. The obtained extract
was incorporated in a base cosmetic cream to ascertain bioactive properties sustainment.
The extract and its corresponding formulation were further submitted to in vitro safety evaluation using MTT and LDH assays in Keratinocyte (HaCaT) and Fibroblast (HFF-1) cell lines and, also in vitro skin permeation studies using a Franz diffusion apparatus with pig ear skin as permeation membrane. Ganoderic acids C2 (38.7±0.2 mg/g), A (36.77±0.04 mg/g) and H (41. 1±0.4 mg/g) were the most abundant triterpenic acids in the extract, and protocatechuic, p-hydroxybenzoic and syringic acids were the identified phenolic acids. Anti-inflammatory activity (EC^, 112±1 [^g/mL), anti-tyrosinase activity (EC^,2.81±0.01 mg/mL) and broad-spectrum antimicrobial activity (MICs up to 20 mg/mL) were observed for the extract. Cell viability of HaCaT and HFF-1 cell were maintained at 100 μg/mL extract exposure, but a -50% decrease was observed in the cell viability of both
cell lines after exposure to the final formulation. The cumulative amounts of triterpenoids and phenolic acids that penetrated the stratum corneum and epidermis-dermis (i.e. target
sites) after 8h detected by HPLC-DAD-ESI/MSn indicate lower mean concentration in the receptor chamber, while a reduction in bioactives concentration in the donor compartment showed that compounds might be retained m skin layers. The results showed promising cosmeceutical properties of the ethanolic extract obtained from G. lucidum.
Description
Keywords
Ganoderma lucidum
Pedagogical Context
Citation
Taofiq, Oludemi; Rodrigues, Francisca; Heleno, Sandrina A.; Calhelha, Ricardo C.; Barros, Lillian; González-Paramás, Ana M.; Barreiro, M.F.; Oliveira, M.B.P.P.; Ferreira, Isabel C.F.R. (2018). Ganoderma lucidum as a promising ingredient for cosmeceutical application: safety assessment and in vitro skin permeation studies. In XXIV Encontro Luso Galego de Química. Porto. ISBN 978-989-8124-24-1