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Ganoderma lucidum as a promising ingredient for cosmeceutical application: safety assessment and in vitro skin permeation studies

2018Conference object Open access
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dc.contributor.authorTaofiq, Oludemi
dc.contributor.authorRodrigues, Francisca
dc.contributor.authorHeleno, Sandrina A.
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorBarros, Lillian
dc.contributor.authorGonzález-Paramás, Ana María
dc.contributor.authorBarreiro, M.F.
dc.contributor.authorOliveira, Beatriz
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2019-01-07T16:39:50Z
dc.date.available2019-01-07T16:39:50Z
dc.date.issued2018
dc.description.abstractAs the global cosmetic market continues to grow steaduily, there is a continuous demand for product diversification resulting m the emergence of products with different claims and functional properties [l]. Because of the rising consciousness and awareness among consumers about the origin, safety and environmental implications of some of the ingredients used in cosmetic formulations, there is an increasing demand for the use of green and more sustainable raw materials during product formulation. Mushrooms are globally consumed not only because of their nutritional properties but also for their medicinal potential and more recently their utilisation as cosmeceutical ingredients due to their richness in several bioactive compounds [l]. In the present study, the Ganoderma lucidum ethanolic extract was evaluated for antioxidant, anti-inflammatory, anti-tyrosmase, antimicrobial and cytotoxic effects, and further characterized m terms of phenolic acids and triterpenoids using HPLC-DAD-ESI/MSn. The obtained extract was incorporated in a base cosmetic cream to ascertain bioactive properties sustainment. The extract and its corresponding formulation were further submitted to in vitro safety evaluation using MTT and LDH assays in Keratinocyte (HaCaT) and Fibroblast (HFF-1) cell lines and, also in vitro skin permeation studies using a Franz diffusion apparatus with pig ear skin as permeation membrane. Ganoderic acids C2 (38.7±0.2 mg/g), A (36.77±0.04 mg/g) and H (41. 1±0.4 mg/g) were the most abundant triterpenic acids in the extract, and protocatechuic, p-hydroxybenzoic and syringic acids were the identified phenolic acids. Anti-inflammatory activity (EC^, 112±1 [^g/mL), anti-tyrosinase activity (EC^,2.81±0.01 mg/mL) and broad-spectrum antimicrobial activity (MICs up to 20 mg/mL) were observed for the extract. Cell viability of HaCaT and HFF-1 cell were maintained at 100 μg/mL extract exposure, but a -50% decrease was observed in the cell viability of both cell lines after exposure to the final formulation. The cumulative amounts of triterpenoids and phenolic acids that penetrated the stratum corneum and epidermis-dermis (i.e. target sites) after 8h detected by HPLC-DAD-ESI/MSn indicate lower mean concentration in the receptor chamber, while a reduction in bioactives concentration in the donor compartment showed that compounds might be retained m skin layers. The results showed promising cosmeceutical properties of the ethanolic extract obtained from G. lucidum.pt_PT
dc.description.sponsorshiplhe authors are gratefiil to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financiai support to CIMO (UID/AGR/00690/2013). This work is funded by the European Agricultural Fund for Rural Development (EAFRD), through the Rural Development Program (PDR2020), withm the scope of Project MicoCoating (PDR2020-101-031472). Francisca Rodrigues is thankful for her postdoc research grant fi-om the project Operação NORTE-01-0145-FEDER- 000011. This work received financiai support from the European Union (FEDER funds through COMPETE), under the Partnership Agreement PT2020, and National Funds (FCT, Foundation for Scie through project LAQV/UID/QUI/50006/2013 and NORTE-07-0124-FEDER-000069 - Food Sdence.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationTaofiq, Oludemi; Rodrigues, Francisca; Heleno, Sandrina A.; Calhelha, Ricardo C.; Barros, Lillian; González-Paramás, Ana M.; Barreiro, M.F.; Oliveira, M.B.P.P.; Ferreira, Isabel C.F.R. (2018). Ganoderma lucidum as a promising ingredient for cosmeceutical application: safety assessment and in vitro skin permeation studies. In XXIV Encontro Luso Galego de Química. Porto. ISBN 978-989-8124-24-1pt_PT
dc.identifier.isbn978-989-8124-24-1
dc.identifier.urihttp://hdl.handle.net/10198/18316
dc.language.isoporpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectGanoderma lucidumpt_PT
dc.titleGanoderma lucidum as a promising ingredient for cosmeceutical application: safety assessment and in vitro skin permeation studiespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00690%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.citation.conferencePlacePorto, Portugalpt_PT
oaire.citation.titleXXIV Encontro Luso Galego de Químicapt_PT
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNameHeleno
person.familyNameCalhelha
person.familyNameBarros
person.familyNameBarreiro
person.familyNameFerreira
person.givenNameSandrina A.
person.givenNameRicardo C.
person.givenNameLillian
person.givenNameFilomena
person.givenNameIsabel C.F.R.
person.identifier469085
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person.identifier.orcid0000-0001-7224-1098
person.identifier.orcid0000-0002-6801-4578
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0002-6844-333X
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridL-5951-2014
person.identifier.ridJ-2172-2014
person.identifier.ridJ-3600-2013
person.identifier.ridL-9802-2014
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id30067731800
person.identifier.scopus-author-id6507978333
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id57188719717
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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