| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 449.74 KB | Adobe PDF |
Advisor(s)
Abstract(s)
Medicinal plants are sources of bioactive compounds with recognized beneficial effects on human health.
An example is Osyris quadripartita, also known as Osyris lanceolata, which is known for its traditional
therapeutic properties in some African countries. The aim of the present study was to evaluate the
antioxidant (free radical scavenging activity, reducing power and lipid peroxidation inhibition), antiinflammatory
(inhibition of NO production in lipopolysaccharide-stimulated RAW 264.7 macrophages)
and cytotoxic (in a panel of human tumor cell lines and in non-tumor porcine liver primary cells)
properties of O. quadripartita, providing a phytochemical characterization of its aqueous extracts and
different organic fractions, by using high-performance liquid chromatography coupled to diode array
detection and electrospray ionisation mass spectrometry (HPLC-DAD-ESI/MS). Twenty-eight individual
phenolic compounds were identified: fifteen flavan-3-ols, six flavones, four flavonols, two phenolic acids
and one flavanone derivative. The most abundant compounds in the ethyl acetate fraction were
(+)-catechin and procyanidin dimer B1 (EC-4,8-C). Quercetin-3-O-rutinoside was the major compound
in both the n-butanol fraction and crude aqueous extract. In most of the samples and assays the
antioxidant activity was higher than the one revealed by the positive control gallic acid, with the highest
antioxidant activity observed in the ethyl acetate fraction. The same fraction also showed the highest
inhibition of NO production and the highest cytotoxicity against MCF-7 and NCI-H460 cell lines. This
study highlights the potential of O. quadripartita fractions rich in phenolic compounds to be used in
pharmaceutical and cosmetic fields.
Description
Keywords
Citation
Rached, Wahiba; Calhelha, Ricardo C.; Fernandes, Ângela; Carvalho, Ana Maria; Bennaceur, Malika; Marouf, Abderrazak; Barros, Lillian; Santos-Buelga, Celestino Ferreira, Isabel C.F.R. (2016). Phytochemical characterization and bioactive properties of Osyris quadripartita Salzm. ex Decne. leaves from Algeria. RSC Advances. ISSN 2046-2069. 6:76, p. 72768-72776
