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Advisor(s)
Abstract(s)
After a period in which synthetic compounds were preferred, there is an increasing
interest m studyüig the bioactive properties of plants and the active principies behind
their therapeutic properties [l]. Polyphenols are the largest and the most widespread
group of secondary metabolites in plants. With a great variety of structures, ranging
from simple compounds with few aromatic rings, such as phenolic acids, to highly
polymeric substances, such as tannins; they are commonly found in both edible and
inedible plants, and have been reported to have multiple biological effects, including
antioxidant activity [2].
Cytinus hypocistis L. is an endophytic parasitic plant occurring in the Mediterranean
region and it was used in a wide variety of traditional healthcare practices, induding
dysentery, tumour of the throat and eye inflammation treatment [3]. Despite these
wealthy traditional uses, its chemical composiüon is largely unknown. Therefore, the
aim of this study was to characterize this plant in terms of polyphenolic composition
and capacity to inhibit lipid peroxidation. Plant specimens were coUected in June 2018
in Castro Daire, Portugal. After lyophilisation, the phenolic compounds were analysed
in four diíFerent hydroethanolic extracts ofC. hypocistis (whole plant, nectar chamber of
thef lower,p etals, ands talks) usinga nH PLC-DAD/ESI-MSs ystem. Seventeenp henolic
compounds were identified, sbcteen hydrolyzable tannins (with trigalloyl-diHHDPglucoside
as the major compound) and one flavan-3-ol ((+)-catechin), being the petals
the sample that presented the highest concentration ofthe identified compounds.
TBARS method was used to evaluate the inhibition of lipid peroxidation, a widely
recognized primary toxicological event caused by the generation offree radicais [4].
In this study, the hydroethanolic extracts ofthe four samples were capable ofpreventing
the formation ofmalondialdehyde (MDA), a secondary product oflipid peroxidation,
in a dose dependent manner. The highest anti-lipid peroxidation activitywas revealed
by the petals extract (342 ng/mL), also exhibiting the highest concentration ofphenolic
compounds. This activity is much better than the one displayed by the standard Trolox.
This study revealed that the extracts might preveni reactive radical species from
damaging biomolecules such as lipids in the cells. These features are behind the health
benefits for humans and the wide use of tannin-containing plant extracts in herbal
medicine [5]. A correlation study will also be performed to understand the influence
ofphenolic compounds in the bioactive properties ofthis plant.
Description
Keywords
Cytinus hypocistis L.
Pedagogical Context
Citation
Silva, Ana Rita; Dias, Maria Inês; Garcia, Pablo A.; Barros, Lillian; Ferreira, Isabel C.F.R. (2018). Cytinus hypocistis L. plant as source of phenolic compounds with anti-lipid peroxidation activity. In XXIV Encontro Luso Galego de Química. Porto. ISBN 978-989-8124-24-1