Advisor(s)
Abstract(s)
Operations for sorting mushrooms at the industrial level usually generate large amounts of bio-residues
not conforming to strict morphological criteria for commercial purposes, even though their biological
content is not compromised. In this context, the present work aimed at evaluating the potential for reutilizing
industrially discarded Agaricus blazei Murill (ABM). Thus, the content of essential nutrients and the
chemical composition were determined, and MTT and LDH assays were used to evaluate the viability and
cell death of Caco-2 and HT29 cell lines of an ethanolic extract prepared from ABM (preliminary safety
tests for nutraceutical applications). The extract was incorporated into a semi-solid base cosmetic cream
and cell viability effects of the extract, and of the final cream formulation, on a keratinocyte cell line
(HaCaT) were studied (preliminary safety tests for cosmeceutical applications). Essential nutrients, such as
proteins and carbohydrates, and a low fat content were determined for ABM. Twenty-two fatty acids were
detected, with polyunsaturated fatty acids (PUFA) (∼53%) being the most abundant fraction. The cell viabilities
of Caco-2 and HT29 cells were maintained up to 100 μg mL−1. After incorporation into the base
cream, a formulation with a pale yellow colour and favourable pH was obtained. The cell viability of
HaCaT cells in the presence of the extract and the final cream formulation was maintained in a concentration
dependent manner, which indicates the safety of this extract for cosmeceutical applications. The
results suggest that ABM residues can be used as an inexpensive and sustainable source of nutraceutical
and cosmeceutical ingredients.
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Pedagogical Context
Citation
Taofiq, Oludemi; Rodrigues, Francisca; Barros, Lillian; Peralta, Rosane M.; Barreiro, Maria F.; Ferreira, Isabel C.F.R.; Oliveira, M. Beatriz P.P. (2019). Agaricus blazei Murrill from Brazil: An ingredient for nutraceutical and cosmeceutical applications. Food and Function. ISSN 2042-6496. 10, p. 565-572