Advisor(s)
Abstract(s)
Propolis is a resin manufactured by bees through the mixture of plant exudates and
waxes with secreted substances from their metabolism, resulting in a complex mixture of natural
substances of which quality depends on the phytogeographic and climatic conditions around the
hive. The present study investigated the contribution of phenolic compounds to the cytotoxic
and anti-inflammatory activities of propolis. The phenolic composition was evaluated by liquid
chromatography with diode-array detection coupled to electrospray ionization tandem mass
spectrometry (LC/DAD/ESI-MSn) analysis after phenolic extraction. The cytotoxicity of the extracts
was checked using human tumor cell lines (MCF7- breast adenocarcinoma, NCI-H460- non-small cell
lung carcinoma, HeLa- cervical carcinoma, HepG2- hepatocellular carcinoma, and MM127- malignant
melanoma), as well as non-tumor cells (a porcine liver primary culture-PLP2). The anti-inflammatory
activity was assessed using the murine macrophage (RAW 264.7) cell line. The results showed a
composition rich in phenolic acids, such as ca eic and p-coumaric acid, as well as flavonoids, such
as pinocembrin, pinobanksin, and pinobanksin-3-O-butyrate. Samples MP2 from Sefrou and MP3
from Moulay Yaâcoub presented a high concentration in phenolic compounds, while MP1 and MP4
from Boulemane and Immouzzer Mermoucha, respectively, showed similar composition with low
bioactivity. The higher concentration of phenolic compound derivatives, which seems to be the
most cytotoxic phenolic class, can explain the pronounced antitumor and anti-inflammatory activity
observed for sample MP2.
Description
Keywords
Anti-inflammatory Cytotoxicity Phenolic compounds Propolis Tumor cells
Citation
Falcão, Soraia I.; Calhelha, Ricardo C.; Touzani, Soumaya; Lyoussi, Badiaâ; Ferreira, Isabel C.F.R.; Vilas-Boas, Miguel (2019). In vitro interactions of moroccan propolis phytochemical’s on human tumor cell lines and anti-inflammatory properties. Biomolecules. ISSN 2218-273X. 9, p. 1-11