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In vitro interactions of moroccan propolis phytochemical’s on human tumor cell lines and anti-inflammatory properties

2019Journal article Open access
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dc.contributor.authorFalcão, Soraia
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorTouzani, Soumaya
dc.contributor.authorLyoussi, Badiâa
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorVilas-Boas, Miguel
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2020-01-06T14:10:25Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2020-01-06T14:10:25Z
dc.date.issued2019
dc.description.abstractPropolis is a resin manufactured by bees through the mixture of plant exudates and waxes with secreted substances from their metabolism, resulting in a complex mixture of natural substances of which quality depends on the phytogeographic and climatic conditions around the hive. The present study investigated the contribution of phenolic compounds to the cytotoxic and anti-inflammatory activities of propolis. The phenolic composition was evaluated by liquid chromatography with diode-array detection coupled to electrospray ionization tandem mass spectrometry (LC/DAD/ESI-MSn) analysis after phenolic extraction. The cytotoxicity of the extracts was checked using human tumor cell lines (MCF7- breast adenocarcinoma, NCI-H460- non-small cell lung carcinoma, HeLa- cervical carcinoma, HepG2- hepatocellular carcinoma, and MM127- malignant melanoma), as well as non-tumor cells (a porcine liver primary culture-PLP2). The anti-inflammatory activity was assessed using the murine macrophage (RAW 264.7) cell line. The results showed a composition rich in phenolic acids, such as ca eic and p-coumaric acid, as well as flavonoids, such as pinocembrin, pinobanksin, and pinobanksin-3-O-butyrate. Samples MP2 from Sefrou and MP3 from Moulay Yaâcoub presented a high concentration in phenolic compounds, while MP1 and MP4 from Boulemane and Immouzzer Mermoucha, respectively, showed similar composition with low bioactivity. The higher concentration of phenolic compound derivatives, which seems to be the most cytotoxic phenolic class, can explain the pronounced antitumor and anti-inflammatory activity observed for sample MP2.
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Program PT2020 for financial support to CIMO (UID/AGR/00690/2013)
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationFalcão, Soraia I.; Calhelha, Ricardo C.; Touzani, Soumaya; Lyoussi, Badiaâ; Ferreira, Isabel C.F.R.; Vilas-Boas, Miguel (2019). In vitro interactions of moroccan propolis phytochemical’s on human tumor cell lines and anti-inflammatory properties. Biomolecules. ISSN 2218-273X. 9, p. 1-11en_EN
dc.identifier.doi10.3390/biom9080315en_EN
dc.identifier.urihttp://hdl.handle.net/10198/20274
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.subjectAnti-inflammatoryen_EN
dc.subjectCytotoxicityen_EN
dc.subjectPhenolic compoundsen_EN
dc.subjectPropolisen_EN
dc.subjectTumor cellsen_EN
dc.titleIn vitro interactions of moroccan propolis phytochemical’s on human tumor cell lines and anti-inflammatory propertiesen_EN
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00690%2F2013/PT
oaire.fundingStream5876
person.familyNameFalcão
person.familyNameCalhelha
person.familyNameFerreira
person.familyNameVilas-Boas
person.givenNameSoraia
person.givenNameRicardo C.
person.givenNameIsabel C.F.R.
person.givenNameMiguel
person.identifier711432
person.identifier144781
person.identifier1693134
person.identifier.ciencia-idD41A-6BED-4CBB
person.identifier.ciencia-idF313-E3CE-554E
person.identifier.ciencia-id9418-CF95-9919
person.identifier.ciencia-idA918-C6FF-81A4
person.identifier.orcid0000-0003-3735-6951
person.identifier.orcid0000-0002-6801-4578
person.identifier.orcid0000-0003-4910-4882
person.identifier.orcid0000-0002-8665-5280
person.identifier.ridJ-2172-2014
person.identifier.ridE-8500-2013
person.identifier.ridI-5949-2013
person.identifier.scopus-author-id14832459200
person.identifier.scopus-author-id6507978333
person.identifier.scopus-author-id36868826600
person.identifier.scopus-author-id6602648497
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
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