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Abstract(s)
Sustainable food options are increasing among the most concerned consumers, in which they seek to
combine new ingredients with potential health benefits [1]. Edible flowers provide new colors, textures
and vibrancy to any dish, and apart from the "glam" factor, they can provide bioactive compounds [2].
In the presentwork, the edible petals of Rosa canina L, gently supplied by the RBR Foods Company
(Portugal), were tested for their cytotoxic activity against tumor cell lines. The assays were conducted
in the hydromethanolic extract and in the lyophilized infusion, being both of them re-dissolved in water
in order to obtain stock solutions at 100 mg/mL for successive dilutions until determination of Glso
values (concentration that inhibits 50% of the net cell growth). Four human tumor cell lines were
tested: MCF-7 (breast adenocarcinoma), NCIH460 (non-small cell lung câncer), HeLa (cervical
carcinoma) and HepG2 (hepatocellular carcinoma). A non-tumour primary cells' culture (assigned as
PLP2) was also prepared from a freshly harvested porcine liver, and used in the bioassays. Ellipticine
was used as the positive contrai. While the hydromethanolic extract inhibited the growth of HeLa (308
pg/mL), the lyophilized infusion inhibited MCF-7 (377 pg/mL), respectively. Both extracts presented
cytotoxicity for HepG2 (297 |jg/mL and 315 pg/mL for hydromethanolic extract and infusion,
respectively). Nane of the extracts showed toxicity towards PLP2. After chemical characterization of
the extracts, flavonoids were the only phenolic compounds present, being quercetin-3-O-glucoside
and kaempferol-3-O-glucoside the major compounds present in both extracts.
These results showed that R. canina edible flowers are sources of bioactive compounds related with
cytotoxic properties in human tumor cell lines, highlighting their applicability potential in nutraceutical
and pharmaceutical fields.
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Citation
Pires, Tânia C.S.; Dias, Maria Inês; Barros, Lillian; Calhelha, Ricardo; Oliveira, M.B.P.P.; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R. (2017). Rosa canina L. as new food ingredient an source of bioactive compounds. In 3rd Symposium on Medicinal Chemistry of University of Minho. Braga