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Advisor(s)
Abstract(s)
An optimized ultrasound-assisted extractive method was developed to obtain a polyphenol-enriched extract from
the aerial parts of Thymus comosus Heuff. ex Griseb. et Schenk. Optimization process was conducted based on
Design of Experiment (DoE) principles, determining the influence of three independent variables (time, ultrasound
amplitude, ethanol concentration) on the total phenolic content of the extract (dependent variable).
Additionally, the phenolic composition of the extract was characterized through UHPLC-HRMS, revealing beside
the most abundant flavonoid-type compounds the presence of salvianolic acids C, D and L in high amounts.
Phytochemical profile of the extract was correlated with its antioxidant activity (tested through five complementary
assays) and enzyme-inhibitory potential, showing important antiglucosidase and anticholinesterase
effects. Overall, it was concluded that the developed method is suitable for obtaining a good recovery of both
phenolic and non-phenolic compounds from Thymus comosus aerial parts, and their presence in the optimized
extract is responsible for its pharmacological potential.
Description
Keywords
Thymus comosus Heuff. ex Griseb. et Schenk Ultrasound-assisted extraction Optimisation Design of Experiments Phenolic compounds Research Subject Categories::FORESTRY, AGRICULTURAL SCIENCES and LANDSCAPE PLANNING::Product science::Food science
Pedagogical Context
Citation
Babotă, Mihai; Frumuzachi, Oleg; Gâvan, Alexandru; Iacoviță, Cristian; Pinela, José; Barros, Lillian; Ferreira, Isabel C.F.R.; Zhang, Leilei; Lucini, Luigi; Rocchetti, Gabriele; Tanase, Corneliu; Crișan, Gianina; Mocan, Andrei (2022). Optimized ultrasound-assisted extraction of phenolic compounds from Thymus comosus Heuff. ex Griseb. et Schenk (wild thyme) and their bioactive potential. Ultrasonics Sonochemistry. ISSN 1350-4177. 84, p. 1-10