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Medicinal plants are known as being a rich source of bioactive components, which can be correlated with
their biological effects. The present study focused on the valorization of the leaves of Osyris quadripartite
Salzm. ex Decne. with recognized folk medicinal uses in some Africa countries, namely Algeria [1, 2].
Therefore, the aim was to perform a phytochemical characterization, regarding phenolic composition, of
O. quadripartita aqueous extracts and two different organic fractions (n-butanol and ethyl acetate), by
using high-performance liquid chromatography coupled to a diode array detection and electrospray
ionisation mass spectrometry (HPLC-DAD–ESI/MS). The separation was achieved with a Waters
Spherisorb S3 ODS-2 C18 (4.6 mm × 150 mm, 3 μm), using a gradient elution, with 0.1% formic acid in
water and acetonitrile. Twenty-eight individual phenolic compounds were identified: fifteen flavan-3-ols,
six flavones, four flavonols, two phenolic acids and one flavanone derivative. The ethyl acetate fraction
presented the highest concentration in phenolic compounds, being (+)-catechin and procyanidin dimer
B1 (EC-4,8-C) the most abundant compounds (110.5 ± 0.3 and 100.5 ± 0.3 mg/g extract, respectively).
The aqueous extract and the n-butanol fraction presented similar contents of phenolic compounds, being
quercetin-3-O-rutinoside the main molecule present (33.8 ± 0.1 and 17.70 ± 0.02 mg/g extract,
respectively). Moreover, the phenolic profiles of all fractions and extracts presented similarities in their
qualitative composition, although some differences were observed, especially in the ethyl acetate
fraction, where no flavone derivatives were present. This study highlights the potential of O. quadripartita
fractions, rich in phenolic compounds, to be used in food, pharmaceutical and cosmetic fields.
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Rached, Wahiba; Calhelha, Ricardo C.; Fernandes, Ângela; Carvalho, Ana Maria; Bennaceur, Malika; Marouf, Abderrazak; Barros, Lillian; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R. (2017). Fingerprint of phenolic compounds in Osyris quadripartite Salzm. ex Decne. from Algeria. In In 10º Encontro Nacional de Cromatografia: livro de resumos. Bragança. ISBN 978-972-745-234-7