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Advisor(s)
Abstract(s)
Eryngium plants have been used for ornamental, agricultural and folk medicinal
purposes [l]. Within this genus, Eryngium viviparum J. Gay is an endemic plant from the
Northwest of Spain and France and Northeast of Portugal that is included in International
Union for Conservation of Nature red list for threatened plants [2]. For this reason, it is
important to apply ex situ biotechnological conservation tools, such as the in vitro culture, to preserve this species, but also take advantage of the high growth rates and yields of
bioactive secondary metabolites to provide high added-value to this species [3]. Phenolic
compounds, among others, have been reported as important compounds in this genus due
to their antioxidant properties [l]. However, as far as we know, the phenolic composition
and antioxidant activity of E. viviparum have never been described, being this the main purpose of this study.
The in vitro culture of E. viviparum explants was established in solidified MS medium, supplemented with indole-3-butyric acid (0. l mg L-l), at 24 ± 2 °C under a 16/8 photoperiod
in a growth chamber. After five weeks, the aerial parts and roots of the established explants
were corrected, freeze-dried and reduced to a fine powder. Hydroethanolic extracts were
then prepared from both samples by solid-liquid extraction and the phenolic profile
was characterized by HPLC-DAD-ESI/MS [4]. The antioxidant activity was evaluated
in vitro for the lipid peroxidation inhibition capacity by the thiobarbituric acid reactive
substances (TBARS) formation inhibition capacity in brain cell homogenates, and for the
antihaemolytic capacity by the oxidative haemolysis inhibition assay (OxHLIA) using
sheep erythrocytes [4].
Eleven phenolic compounds were identified m both E. viviparum hydroethanolic extracts,
corresponding to ten phenolic acids and one flavonoid. Rosmarinic acid was the major
compound in both samples and the highest concentration was found in roots. Tectorigenin-O-glucuronide was the identified flavonoid. The root extract had an interesting antioxidant
activity translated by the lowest IC values. In fact, the root extract was more effective in preventing the formation of malondialdehyde as a secondary product of lipid peroxidation and preventing lysis of erythrocyte membranes. The high levels of rosmarinic acid found
in roots may be responsible for the observed bioactivities, since this compound has been
reported as a powerful antioxidant [5]. Therefore, this study provided high added-value to E. viviparum roots as an interesting source of rosmarinic acid, which could have different industrial applications as natural antioxidants.
Description
Keywords
Eryngium viviparum
Citation
Ayuso, Manuel; Pinela, José; Dias, Maria Inês; Gallego, Pedro Pablo; Barreal, M. Esther; Barros, Lillian; Ferreira, Isabel C.F.R. (2018). Phenolic composition and cell-based antioxidant activity of roots and aerial parts of Eryngium viviparum produced in vitro. In XXIV Encontro Luso Galego de Química. Porto. ISBN Ayuso, Manuel; Pinela, José; Dias, Maria Inês; Gallego, Pedro Pablo; Barreal, M. Esther; Barros, Lillian; Ferreira, Isabel C.F.R. (2018). Phenolic composition and cell-based antioxidant activity of roots and aerial parts of Eryngium viviparum produced in vitro. In XXIV Encontro Luso Galego de Química. Porto. ISBN 978-989-8124-24-1