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Phenolic composition and cell-based antioxidant activity of roots and aerial parts of Eryngium viviparum produced in vitro

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dc.contributor.authorAyuso, Manuel
dc.contributor.authorPinela, José
dc.contributor.authorDias, Maria Inês
dc.contributor.authorGallego, Pedro Pablo
dc.contributor.authorBarreal, M. Esther
dc.contributor.authorBarros, Lillian
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2019-01-07T12:08:22Z
dc.date.available2019-01-07T12:08:22Z
dc.date.issued2018
dc.description.abstractEryngium plants have been used for ornamental, agricultural and folk medicinal purposes [l]. Within this genus, Eryngium viviparum J. Gay is an endemic plant from the Northwest of Spain and France and Northeast of Portugal that is included in International Union for Conservation of Nature red list for threatened plants [2]. For this reason, it is important to apply ex situ biotechnological conservation tools, such as the in vitro culture, to preserve this species, but also take advantage of the high growth rates and yields of bioactive secondary metabolites to provide high added-value to this species [3]. Phenolic compounds, among others, have been reported as important compounds in this genus due to their antioxidant properties [l]. However, as far as we know, the phenolic composition and antioxidant activity of E. viviparum have never been described, being this the main purpose of this study. The in vitro culture of E. viviparum explants was established in solidified MS medium, supplemented with indole-3-butyric acid (0. l mg L-l), at 24 ± 2 °C under a 16/8 photoperiod in a growth chamber. After five weeks, the aerial parts and roots of the established explants were corrected, freeze-dried and reduced to a fine powder. Hydroethanolic extracts were then prepared from both samples by solid-liquid extraction and the phenolic profile was characterized by HPLC-DAD-ESI/MS [4]. The antioxidant activity was evaluated in vitro for the lipid peroxidation inhibition capacity by the thiobarbituric acid reactive substances (TBARS) formation inhibition capacity in brain cell homogenates, and for the antihaemolytic capacity by the oxidative haemolysis inhibition assay (OxHLIA) using sheep erythrocytes [4]. Eleven phenolic compounds were identified m both E. viviparum hydroethanolic extracts, corresponding to ten phenolic acids and one flavonoid. Rosmarinic acid was the major compound in both samples and the highest concentration was found in roots. Tectorigenin-O-glucuronide was the identified flavonoid. The root extract had an interesting antioxidant activity translated by the lowest IC values. In fact, the root extract was more effective in preventing the formation of malondialdehyde as a secondary product of lipid peroxidation and preventing lysis of erythrocyte membranes. The high levels of rosmarinic acid found in roots may be responsible for the observed bioactivities, since this compound has been reported as a powerful antioxidant [5]. Therefore, this study provided high added-value to E. viviparum roots as an interesting source of rosmarinic acid, which could have different industrial applications as natural antioxidants.pt_PT
dc.description.sponsorshipTo FCT (Portugal) and FEDER under programme PT2020 for the financiai support to CIMO (UID/AGR/00690/2013) and the research contracts ofj. Pinela (Project AllNatt, POCI- 01-0145-FEDER-030463) and L. Barras. To FEDER-Interreg Espana-Portugal programme for financiai support through the project 0377_Iberphenol_6_E. To the University ofVigo for the M. Ayuso stay grant in research centres. To the Xunta de Galicia for financiai support (Programa REDES ED431D-2017/18).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAyuso, Manuel; Pinela, José; Dias, Maria Inês; Gallego, Pedro Pablo; Barreal, M. Esther; Barros, Lillian; Ferreira, Isabel C.F.R. (2018). Phenolic composition and cell-based antioxidant activity of roots and aerial parts of Eryngium viviparum produced in vitro. In XXIV Encontro Luso Galego de Química. Porto. ISBN Ayuso, Manuel; Pinela, José; Dias, Maria Inês; Gallego, Pedro Pablo; Barreal, M. Esther; Barros, Lillian; Ferreira, Isabel C.F.R. (2018). Phenolic composition and cell-based antioxidant activity of roots and aerial parts of Eryngium viviparum produced in vitro. In XXIV Encontro Luso Galego de Química. Porto. ISBN 978-989-8124-24-1pt_PT
dc.identifier.isbn978-989-8124-24-1
dc.identifier.urihttp://hdl.handle.net/10198/18310
dc.language.isoporpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEryngium viviparum
dc.titlePhenolic composition and cell-based antioxidant activity of roots and aerial parts of Eryngium viviparum produced in vitropt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00690%2F2013/PT
oaire.citation.conferencePlacePorto, Portugalpt_PT
oaire.citation.titleXXIV Encontro Luso Galego de Químicapt_PT
oaire.fundingStream5876
person.familyNameAyuso
person.familyNamePinela
person.familyNameDias
person.familyNameBarros
person.familyNameFerreira
person.givenNameManuel
person.givenNameJosé
person.givenNameMaria Inês
person.givenNameLillian
person.givenNameIsabel C.F.R.
person.identifierABC-7764-2020
person.identifier469085
person.identifier144781
person.identifier.ciencia-id6511-A057-C55F
person.identifier.ciencia-id771C-2B43-B108
person.identifier.ciencia-id2A13-4BE6-C7CF
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0002-1697-3857
person.identifier.orcid0000-0001-7523-1637
person.identifier.orcid0000-0001-8744-7814
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridB-4466-2014
person.identifier.ridM-8242-2013
person.identifier.ridJ-3600-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id54392272800
person.identifier.scopus-author-id54388787000
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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