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Advisor(s)
Abstract(s)
Anthocyanin compounds are pigments that have a red, blue, and/or violet coloration widely found in nature,
mainly in fruits [1]. Given the current need to replace synthetic colorants with healthier natural alternatives,
this study investigated the potential of haskap (Prunus spinosa L.) and blackthorn (Lonicera careulea L.)
fruits as sources of anthocyanins. For this purpose, the characterization of these fruits (the hydroethanolic
extract of blachthorn epicarp and the haskap juice) was carried out by high performance liquid
chromatography coupled to a diode array detector and a mass spectrometer (HPLC-DAD/ESI-MS). The
identification was carried out using standards, when available, comparing their retention times, UV-Vis
spectra, and mass spectra. In the absence of standards, the identification was carried out by the fragmentation
profile and by comparison with the information available in the literature. The quantification was performed
from the peak areas recorded at a wavelength of 520 nm, compared to the standard calibration curves.
The blackthorn epicarp showed two anthocyanin compounds, being the molecules present in the highest
concentrations cyanidin-3-O-rutinoside ([H]+ at m/z 595) and peonidin-3-O-rutinoside ([H]+ at m/z 609). In
haskap berries, six anthocyanins were identified, namely cyanidin-O-hexoside-O-hexoside ([H]+ at m/z 611),
cyanidin-O-rhamnoside-O-hexoside ([H]+ at m/z 595), pelargonidin-3-O-glucoside ([H]+ at m/z 433),
peonidin-3-O-glucoside ([H]+ at m/z 463), peonidin-O-rhamnoside-O-hexoside ([H]+ at m/z 609), and
cyanidin-3-O-glucoside ([H]+ at m/z 449), with the latest as the most abundant one. These results
demonstrate that the blackthorn fruit epicarp and haskap fruits can be considered great sources of pigments
in the red-purple colour range, having applicability both in the food and pharmaceutical industries.
Description
Keywords
Anthocyanin compounds Prunus spinosa L. Lonicera careulea L.
Pedagogical Context
Citation
Leichtweis, Maria Gabriela; Molina, Adriana K.; Pereira, Carla; Dias, Maria Inês; Ferreira, Isabel C.F.R.; Barros, Lillian (2022). Haskap and blackthorn berries anthocyanin profile. In 1st Research Meeting on Biochemistry. Maringá