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Haskap and blackthorn berries anthocyanin profile

dc.contributor.authorLeichtweis, Maria Gabriela
dc.contributor.authorMolina, Adriana K.
dc.contributor.authorPereira, Carla
dc.contributor.authorDias, Maria Inês
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorBarros, Lillian
dc.date.accessioned2022-07-21T10:23:36Z
dc.date.available2022-07-21T10:23:36Z
dc.date.issued2022
dc.description.abstractAnthocyanin compounds are pigments that have a red, blue, and/or violet coloration widely found in nature, mainly in fruits [1]. Given the current need to replace synthetic colorants with healthier natural alternatives, this study investigated the potential of haskap (Prunus spinosa L.) and blackthorn (Lonicera careulea L.) fruits as sources of anthocyanins. For this purpose, the characterization of these fruits (the hydroethanolic extract of blachthorn epicarp and the haskap juice) was carried out by high performance liquid chromatography coupled to a diode array detector and a mass spectrometer (HPLC-DAD/ESI-MS). The identification was carried out using standards, when available, comparing their retention times, UV-Vis spectra, and mass spectra. In the absence of standards, the identification was carried out by the fragmentation profile and by comparison with the information available in the literature. The quantification was performed from the peak areas recorded at a wavelength of 520 nm, compared to the standard calibration curves. The blackthorn epicarp showed two anthocyanin compounds, being the molecules present in the highest concentrations cyanidin-3-O-rutinoside ([H]+ at m/z 595) and peonidin-3-O-rutinoside ([H]+ at m/z 609). In haskap berries, six anthocyanins were identified, namely cyanidin-O-hexoside-O-hexoside ([H]+ at m/z 611), cyanidin-O-rhamnoside-O-hexoside ([H]+ at m/z 595), pelargonidin-3-O-glucoside ([H]+ at m/z 433), peonidin-3-O-glucoside ([H]+ at m/z 463), peonidin-O-rhamnoside-O-hexoside ([H]+ at m/z 609), and cyanidin-3-O-glucoside ([H]+ at m/z 449), with the latest as the most abundant one. These results demonstrate that the blackthorn fruit epicarp and haskap fruits can be considered great sources of pigments in the red-purple colour range, having applicability both in the food and pharmaceutical industries.pt_PT
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to CIMO (UIDB/00690/2020); national funding by FCT, P.I., through the institutional scientific employment program-contract for C. Pereira and L. Barros contracts and A.K. Molina and M.G. Leichtweis PhD grants (2020.06231.BD and 2020.06706.BD, respectively). To FEDER-Interreg España-Portugal programme for financial support through TRANSCoLAB 0612_TRANS_CO_LAB_2_P project; to the European Regional Development Fund (ERDF) through the Regional Operational Program North 2020, within the scope of Project Mobilizador Norte-01-0247-FEDER-024479: ValorNatural®pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLeichtweis, Maria Gabriela; Molina, Adriana K.; Pereira, Carla; Dias, Maria Inês; Ferreira, Isabel C.F.R.; Barros, Lillian (2022). Haskap and blackthorn berries anthocyanin profile. In 1st Research Meeting on Biochemistry. Maringápt_PT
dc.identifier.urihttp://hdl.handle.net/10198/25707
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationMountain Research Center
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnthocyanin compoundspt_PT
dc.subjectPrunus spinosa L.pt_PT
dc.subjectLonicera careulea L.pt_PT
dc.titleHaskap and blackthorn berries anthocyanin profilept_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.citation.conferencePlaceMaringá, Paraná, Brasilpt_PT
oaire.citation.title1st Research Meeting on Biochemistrypt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameLeichtweis
person.familyNameMolina
person.familyNamePereira
person.familyNameDias
person.familyNameFerreira
person.familyNameBarros
person.givenNameMaria Gabriela
person.givenNameAdriana K.
person.givenNameCarla
person.givenNameMaria Inês
person.givenNameIsabel C.F.R.
person.givenNameLillian
person.identifier1415151
person.identifier144781
person.identifier469085
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person.identifier.ciencia-idC110-0167-E58D
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person.identifier.ciencia-id9418-CF95-9919
person.identifier.ciencia-id9616-35CB-D001
person.identifier.orcid0000-0002-6015-9043
person.identifier.orcid0000-0002-6234-2212
person.identifier.orcid0000-0003-0093-771X
person.identifier.orcid0000-0001-8744-7814
person.identifier.orcid0000-0003-4910-4882
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridK-1629-2016
person.identifier.ridM-8242-2013
person.identifier.ridE-8500-2013
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id55627876424
person.identifier.scopus-author-id54388787000
person.identifier.scopus-author-id36868826600
person.identifier.scopus-author-id35236343600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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