Advisor(s)
Abstract(s)
An ultrasound rapid and low-cost procedure for anthocyanin-based colorants from
Prunus spinosa L. fruit epicarp was developed, and the advantages were compared with conventional
heat-based extraction. To obtain the conditions that maximize anthocyanins’ extraction, a response
surface methodology was applied using the variables of time, temperature, and ethanol content,
in the case of heat extraction, whereas for ultrasound assisted extraction, temperature was replaced by
ultrasound power. Two anthocyanin compounds were identified by HPLC-DAD-ESI/MS—namely,
cyanidin 3-rutinoside and peonidin 3-rutinoside. The responses used were the extraction yield and
the content of the identified anthocyanins. Ultrasound extraction was the most effective method
at 5.00 0.15 min, 400.00 32.00 W, and 47.98% 2.88% of ethanol obtaining 68.60% 2.06% of
extracted residue, with an anthocyanin content of 18.17 mg/g (extract-basis) and 11.76 mg/g
(epicarp-basis). Overall, a viable green process was achieved that could be used to support pilot-scale
studies for industrial production of anthocyanin-based colorants from P. spinosa fruit epicarp.
Description
Keywords
Cyanidin 3-rutinoside Heat and ultrasound assisted extraction Peonidin 3-rutinoside Prunus spinosa L. fruit epicarp Response surface methodology Wild fruit valorization
Pedagogical Context
Citation
Leichtweis, Maria G.; Pereira, Carla; Prieto, M. A.; Barreiro, Maria Filomena; Baraldi, Ilton José; Barros, Lillian; Ferreira, Isabel C.F.R. (2019). Ultrasound as a Rapid and Low-Cost Extraction Procedure to Obtain Anthocyanin-Based Colorants from Prunus spinosa L. Fruit epicarp: Comparative study with conventional heat-based extraction. Molecules. ISSN 1420-3049. 24, p. 1-17