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Authors
Advisor(s)
Abstract(s)
The phytochemical compounds of Calendula officinalis leaf are the subject of
various histochemical tests. The scientific literature describes the presence of several
compounds of interest. Identify groups of compounds from secondary metabolism with
histochemical staining techniques of Calendula officinalis leaf. Histochemical staining
techniques following pre-established protocols for detection of lipids in general (Sudans),
Fatty Acids (Copper Acetate / Rubric Acid), Terpenoids (Nadi), Proteins in general,
Phenols (Iron Trichloride), Alkaloids (Dittmar), carbohydrates (Periodic Acid / Schiff
Reagent), tannins (Hydrochloric Vanillin), and observation and identification by optical
microscope. In the trichomes and in the parenchyma are some lipids detected. Free fatty
acids are detected at the parenchyma. Terpenoids are exclusively detected at the
trichomes. Phenols are detected at the parenchyma. A few amounts of alkaloids are
detected at the trichomas. There is no detection of proteins by Sakaguchi reaction for
arginine. The presents of carbohydrates is not strongly confirmed. The present of tannins
is in general confirmed. The performed tests shown that the leaves of the Calendula
officinalis plant contain several types of compounds of secondary metabolism, which
could be of interest for further investigation, in particular because the plant is easily be
grown in large quantities and in a large distribution area.
Keywords: Calendula officinalis leaf; Histochemical tests; Phenols; Alkaloids;
Terpenoid
Description
Keywords
Calendula officinalis leaf Histochemical tests Phenols Alkaloids Terpenoids
Citation
Kautzmann, B.; Sousa, Maria João (2019). Histochemical tests in Calendula officinalis leaf. In Livro de Resumos do XXIV Encontro Luso-Galego de Química