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Histochemical tests in Calendula officinalis leaf

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dc.contributor.authorKautzmann, B.
dc.contributor.authorSousa, Maria João
dc.date.accessioned2020-06-16T14:30:52Z
dc.date.available2020-06-16T14:30:52Z
dc.date.issued2019
dc.description.abstractThe phytochemical compounds of Calendula officinalis leaf are the subject of various histochemical tests. The scientific literature describes the presence of several compounds of interest. Identify groups of compounds from secondary metabolism with histochemical staining techniques of Calendula officinalis leaf. Histochemical staining techniques following pre-established protocols for detection of lipids in general (Sudans), Fatty Acids (Copper Acetate / Rubric Acid), Terpenoids (Nadi), Proteins in general, Phenols (Iron Trichloride), Alkaloids (Dittmar), carbohydrates (Periodic Acid / Schiff Reagent), tannins (Hydrochloric Vanillin), and observation and identification by optical microscope. In the trichomes and in the parenchyma are some lipids detected. Free fatty acids are detected at the parenchyma. Terpenoids are exclusively detected at the trichomes. Phenols are detected at the parenchyma. A few amounts of alkaloids are detected at the trichomas. There is no detection of proteins by Sakaguchi reaction for arginine. The presents of carbohydrates is not strongly confirmed. The present of tannins is in general confirmed. The performed tests shown that the leaves of the Calendula officinalis plant contain several types of compounds of secondary metabolism, which could be of interest for further investigation, in particular because the plant is easily be grown in large quantities and in a large distribution area. Keywords: Calendula officinalis leaf; Histochemical tests; Phenols; Alkaloids; Terpenoidpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationKautzmann, B.; Sousa, Maria João (2019). Histochemical tests in Calendula officinalis leaf. In Livro de Resumos do XXIV Encontro Luso-Galego de Químicapt_PT
dc.identifier.urihttp://hdl.handle.net/10198/22070
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectCalendula officinalis leafpt_PT
dc.subjectHistochemical testspt_PT
dc.subjectPhenolspt_PT
dc.subjectAlkaloidspt_PT
dc.subjectTerpenoidspt_PT
dc.titleHistochemical tests in Calendula officinalis leafpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceViseupt_PT
oaire.citation.titleComissão organizadora do III Congresso Nacional das Escolas Superiores Agráriaspt_PT
person.familyNameSousa
person.givenNameMaria João
person.identifier.ciencia-idD91E-3571-3979
person.identifier.orcid0000-0002-9946-4926
person.identifier.scopus-author-id55230282700
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationda5c7615-9844-4fdf-b4d4-64d0c8e72de0
relation.isAuthorOfPublication.latestForDiscoveryda5c7615-9844-4fdf-b4d4-64d0c8e72de0

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