Browsing by Author "Rached, Wahiba"
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- Bioactive properties of the endemic algerian Myrtus nivelii Batt & Trab.: scientific approaches to the traditional usesPublication . Rached, Wahiba; Bennaceur, Malika; Barros, Lillian; Calhelha, Ricardo C.; Heleno, Sandrina A.; Alves, Maria José; Carvalho, Ana Maria; Marouf, Abderrazak; Ferreira, Isabel C.F.R.Myrtus nivelli ileaves are widely used in the Algerian folk medicine, due toits healthpromoting properties attributed to the phenolic composition. In this context, bioactivities of the aqueous extract and its organic sub-fractions (ethyl acetate and butanol) were evaluated with focus onantioxidant properties (free radicals’ scavenging activity, reducing power, inhibition of β-carotene blanching andlipid peroxidation), anti-inflammatory potential. (inhibition of NO production in lipopolysaccharide-stimulated RAW 264.7 macrophages), cytotoxicity for human tumor cells and normal porcine liver primary cells, and antibacterial activity against ten clinical isolates. The obtained results indicated high activities for all the tested samples. The antioxidant activity was higher than that obtained for Trolox (positive control), showing the ethylacetate fraction the highest activity as also the highest inhibition of NO production (EC50= 104 ± 6 μg/mL) and cytotoxicity for all the tested tumor cell lines (HeLa- 15.39±1 μg/mL, MCF-7- 16.12± 1μg/mL, NCI-H460-, HepG2-17.67±1 μg/mL and 49.01 ± 3 μg/mL). The butanol fraction and the crude aqueous extract presented the highest antibacterial activity. Overall, this study highlights the potential of M. nivellii leaves in the preparation of bioactive ingredients.
- Detailed chemical composition and functional properties of Ammodaucus leucotrichus Cross. & Dur. and Moringa oleifera LamarckPublication . Ziani, Borhane E.C.; Rached, Wahiba; Bachari, Khaldoun; Alves, Maria José; Calhelha, Ricardo C.; Barros, Lillian; Ferreira, Isabel C.F.R.Edible Ammodaucus leucotrichus Cross. & Dur. and Moringa oleifera Lamarck are largely used in the Algerian Sahara. In this context, soluble sugars, fatty acids, organic acids, and tocopherols were characterized. Decoctions and hydroethanolic extracts were studied regarding their phenolic compounds by HPLC-DAD-ESI/MSn, and in-vitro cytotoxic, anti-inflammatory, and antibacterial activities were evaluated. Results indicate high contents of carbohydrates (glucose and sucrose) and significant levels of protein and mineral were recorded for both plants. Malic acid was the major organic acid alongside with α-tocopherol, while PUFA was predominant in M. oleifera and MUFA in A. leucotrichus. Flavonoid derivatives were the most abundant group, being luteolin-O-(malonyl-glucoside) the main compound in A. leucotrichus and quercetin-3-O-(6″-malonyl-glucoside) and 3-O-caffeoylquininc acid the major molecules in M. oleifera. Extracts exhibited significant cytotoxicity on Hela and MCF-7 cell lines. The anti-inflammatory activity was also higher in the hydroethanolic extracts, which also revealed the highest antibacterial effects, especially for Gram-positive bacteria.
- Detailed phytochemical characterization and bioactive properties of: Myrtus nivelii Batt & TrabPublication . Rached, Wahiba; Bennaceur, Malika; Barros, Lillian; Calhelha, Ricardo C.; Heleno, Sandrina A.; Alves, Maria José; Carvalho, Ana Maria; Marouf, Abderrazak; Ferreira, Isabel C.F.R.The endemic Algerian myrtle, Myrtus nivelii Batt & Trab., was evaluated for its bioactive properties, such as antioxidant, anti-inflammatory, cytotoxic and antibacterial activities, and correlated with the individual phenolic compounds identified in its crude aqueous extract and subsequent organic fractions (ethyl acetate and butanol). Flavonols, such as myricetin, kaempferol and quercetin glycoside derivatives, were the major phenolic compounds found, along with the presence of ellagitannins. The ethyl acetate fraction contained the highest amount of phenolic compounds, followed by the butanol fraction and, finally, the crude aqueous extract. In general, all samples exhibited an excellent bioactivity, namely the ethyl acetate fraction that presented strong antioxidant activity, when compared to the standard trolox, strong cytotoxicity and antibacterial activity, especially against MRSA and MSSA. The present study revealed that the ethyl acetate fraction of M. nivelii could be used as a source of bioactive compounds in the food and pharmaceutical industries.
- Fingerprint of phenolic compounds in Osyris quadripartite Salzm. ex Decne. from AlgeriaPublication . Rached, Wahiba; Calhelha, Ricardo C.; Fernandes, Ângela; Carvalho, Ana Maria; Bennaceur, Malika; Marouf, Abderrazak; Barros, Lillian; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R.Medicinal plants are known as being a rich source of bioactive components, which can be correlated with their biological effects. The present study focused on the valorization of the leaves of Osyris quadripartite Salzm. ex Decne. with recognized folk medicinal uses in some Africa countries, namely Algeria [1, 2]. Therefore, the aim was to perform a phytochemical characterization, regarding phenolic composition, of O. quadripartita aqueous extracts and two different organic fractions (n-butanol and ethyl acetate), by using high-performance liquid chromatography coupled to a diode array detection and electrospray ionisation mass spectrometry (HPLC-DAD–ESI/MS). The separation was achieved with a Waters Spherisorb S3 ODS-2 C18 (4.6 mm × 150 mm, 3 μm), using a gradient elution, with 0.1% formic acid in water and acetonitrile. Twenty-eight individual phenolic compounds were identified: fifteen flavan-3-ols, six flavones, four flavonols, two phenolic acids and one flavanone derivative. The ethyl acetate fraction presented the highest concentration in phenolic compounds, being (+)-catechin and procyanidin dimer B1 (EC-4,8-C) the most abundant compounds (110.5 ± 0.3 and 100.5 ± 0.3 mg/g extract, respectively). The aqueous extract and the n-butanol fraction presented similar contents of phenolic compounds, being quercetin-3-O-rutinoside the main molecule present (33.8 ± 0.1 and 17.70 ± 0.02 mg/g extract, respectively). Moreover, the phenolic profiles of all fractions and extracts presented similarities in their qualitative composition, although some differences were observed, especially in the ethyl acetate fraction, where no flavone derivatives were present. This study highlights the potential of O. quadripartita fractions, rich in phenolic compounds, to be used in food, pharmaceutical and cosmetic fields.
- Green and Innovative Extraction: Phenolic Profiles and Biological Activities of Underutilized Plant Extracts Using Pulsed Electric Fields and MacerationPublication . Pallarés, Noelia; Berrada, Houda; Ferrer, Emilia; Rached, Wahiba; Pinela, José; Mandim, Filipa; Pires, Tânia C.S.P.; Finimundy, Tiane C.; Barba, Francisco J.; Barros, LillianUnderutilized plant species such as Asteriscus graveolens (Forssk.) Less., Haloxylon scoparium Pomel, and Ruta chalepensis L. have been historically valued in traditional medicine for their potential health benefits. These species present an untapped source of bioactive compounds with significant applications in the food and pharmaceutical industries, including the development of functional foods and additives. Recent advances in food processing have introduced innovative methods, such as pulsed electric fields (PEFs), to enhance the extraction of valuable compounds without compromising their integrity or quality. This study investigates the impact of PEF technology on the recovery of bioactive compounds from these plants, comparing it with conventional maceration (MAC) techniques. Phenolic compound profiles and biological activities, including antioxidant, antimicrobial, anti-inflammatory, and cytotoxic effects, were evaluated. The results demonstrated that for R. chalepensis, PEF extraction achieved comparable phenolic content (58 mg/g) to MAC (72 mg/g). However, MAC generally provided higher phenolic yields for other plants. A. graveolens extracts exhibited significant antitumoral and anti-inflammatory potentials. The antimicrobial results indicated that MAC extracts were more effective against bacterial growth, while PEF extracts outperformed MAC against A. brasiliensis (MIC: 10 mg/mL). Antioxidant potential was observed in both methods, with TBARS IC50 values ranging from 17 to 79.5 µg/mL. While MAC generally yielded superior results, PEF extraction showed great promise as an environmentally sustainable alternative, eliminating the need for organic solvents and aligning with green extraction principles.
- HPLC-DAD-ESI-MS/MS screening of phytochemical compounds and the bioactive properties of different plant parts of: Zizyphus lotus (L.) Desf.Publication . Rached, Wahiba; Barros, Lillian; Ziani, Borhane E.C.; Bennaceur, Malika; Calhelha, Ricardo C.; Heleno, Sandrina A.; Alves, Maria José; Marouf, Abderrazak; Ferreira, Isabel C.F.R.Zizyphus lotus L. (Desf.) has been widely used as a homemade ingredient to treat numerous diseases in Algerian folk medicine. In this context, decoction, infusion and hydroethanolic extracts were prepared from the branches, leaves, roots, and stem barks. The extracts were characterized regarding their phytochemical composition by HPLC-DAD-ESI/MSn and were assessed for in vitro bioactivity (cytotoxicity, anti-inflammatory activity, and antibacterial activity). A total of 29 compounds (flavonoid derivatives and secoiridoids) were identified in Z. lotus, 15 being detected in leaves, 10 in branches, 5 in roots and 7 in stem barks. The highest contents of these compounds, especially secoiridoids, were detected in branches followed by leaves, oleuropein being the main compound detected in leaves and oleoside in branches. The aqueous preparations of the leaves and root barks revealed the highest cytotoxicity and anti-inflammatory potential. On the other hand, as for the antibacterial activity, the hydroethanolic extract of the branches showed the highest potential, especially against the MSSA strain. Hence, the chemical diversity found in Z. lotus makes it a source of bioactive ingredients that can be applied in the formulations of different ingredients in the food and pharmaceutical industries.
- Phenolic profile and in vitro bioactive potential of Saharan Juniperus phoenicea L. and Cotula cinerea (Del) growing in AlgeriaPublication . Ghouti, Dalila; Rached, Wahiba; Abdallah, Moussaoui; Pires, Tânia C.S.; Calhelha, Ricardo C.; Alves, Maria José; Abderrahmane, Lazzouni Hamadi; Barros, Lillian; Ferreira, Isabel C.F.R.The aim of this study was to characterize the individual phenolic profile and antioxidant, anti-inflammatory, cytotoxic, and antimicrobial activities of hydroethanolic and infusion extracts prepared from Algerian Saharan Juniperus phoenicea L. and Cotula cinerea (Del). The phenolic profile was determined using a liquid chromatograph coupled to a diode array detector and an electrospray ionization mass spectrometer (LC-DAD-ESI/MS). A total of thirteen and nine individual phenolic compounds were identified in J. phoenicea and C. cinerea, respectively. 3-p-Coumaroylquinic acid, quercetin- and myricetin-O-pentoside were the major compounds present in J. phoenicea; on the other hand, C. cinerea presented luteolin-7-O-glucoside, luteolin-O-malonylhexoside, and 5-O-caffeoylquinic acid as the main molecules. In general, all samples exhibited interesting antioxidant activity when compared to the standard Trolox, but J. phoenicea extracts presented the highest bioactivity. Likewise, all the samples exhibited anti-inflammatory activity; thus J. phoenicea hydroethanolic extracts showed the highest potential (88 ± 8 µg mL−1). In addition, their cytotoxicity was evaluated towards a panel of four selected cell lines (HeLa, NCI-H460, MCF-7 and HepG2), and all the extracts showed cytotoxic effects, with J. phoenicea extracts being the most effective. The in vitro antimicrobial activity of the plant extracts was moderate, Gram-positive bacteria thus being more sensitive than the Gram-negative strains (MIC values between 5 and 20 mg mL−1). The present work suggests that J. phoenicea and C. cinerea are sources of bioactive ingredients with potential use in the food and pharmaceutical industries.
- Phytochemical analysis and assessment of antioxidant, antimicrobial, anti-inflammatory and cytotoxic properties of Tetraclinis articulata (Vahl) Masters leavesPublication . Rached, Wahiba; Zeghada, Fatima Zohra; Bennaceur, Malika; Barros, Lillian; Calhelha, Ricardo C.; Heleno, Sandrina A.; Alves, Maria José; Carvalho, Ana Maria; Marouf, Abderrazak; Ferreira, Isabel C.F.R.Tetraclinis is an unexplored genus recognized for its great bioactive potential that could be explored as a medicinal herb. The aim of this study was to evaluate the antioxidant, antibacterial, anti-inflammatory and cytotoxic properties of the aqueous crude extract and subsequent organic fractions of the Algerian medicinal plant T. articulata. Moreover, polyphenols were also fully profiled by liquid chromatography with diode-array detection coupled to electrospray ionization in tandem with mass spectrometry (LC–DAD–ESI–MSn). The analysis of the obtained results show that B-type (epi)catechin dimer and catechin were the most abundant phenolic compounds present among the nine different flavonoids identified such as: ((epi)catechin, myricetin, quercetin and kaempferol glycoside derivatives). The concentration of phenolic compounds in the ethyl acetate fraction (93.1 mg/g extract) was higher than the one of the aqueous extract (21.2 mg/g extract) and butanol fraction (43.87 mg/g extract). Furthermore, the ethyl acetate fraction revealed the strongest bioactive properties, revealing the lowest EC50/GI50 values: anti-inflammatory (EC50 129.67 μg/mL), cytotoxicity against human tumor cell lines (GI50, 59–189 μg/mL), antibacterial (MIC, 0.625–10 mg/mL); and especially the antioxidant capacity (EC50 values lower than 12.7 μg/mL), even higher than the standard (Trolox). The results showed a great bioactive potential for this species, with a significant contribution of flavonoid compounds, which makes it an interesting matrix in the development of novel pharmaceutical formulations.
- Phytochemical characterization and bioactive properties of Osyris quadripartita Salzm. ex Decne. leaves from AlgeriaPublication . Rached, Wahiba; Calhelha, Ricardo C.; Fernandes, Ângela; Carvalho, Ana Maria; Bennaceur, Malika; Marouf, Abderrazak; Barros, Lillian; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R.Medicinal plants are sources of bioactive compounds with recognized beneficial effects on human health. An example is Osyris quadripartita, also known as Osyris lanceolata, which is known for its traditional therapeutic properties in some African countries. The aim of the present study was to evaluate the antioxidant (free radical scavenging activity, reducing power and lipid peroxidation inhibition), antiinflammatory (inhibition of NO production in lipopolysaccharide-stimulated RAW 264.7 macrophages) and cytotoxic (in a panel of human tumor cell lines and in non-tumor porcine liver primary cells) properties of O. quadripartita, providing a phytochemical characterization of its aqueous extracts and different organic fractions, by using high-performance liquid chromatography coupled to diode array detection and electrospray ionisation mass spectrometry (HPLC-DAD-ESI/MS). Twenty-eight individual phenolic compounds were identified: fifteen flavan-3-ols, six flavones, four flavonols, two phenolic acids and one flavanone derivative. The most abundant compounds in the ethyl acetate fraction were (+)-catechin and procyanidin dimer B1 (EC-4,8-C). Quercetin-3-O-rutinoside was the major compound in both the n-butanol fraction and crude aqueous extract. In most of the samples and assays the antioxidant activity was higher than the one revealed by the positive control gallic acid, with the highest antioxidant activity observed in the ethyl acetate fraction. The same fraction also showed the highest inhibition of NO production and the highest cytotoxicity against MCF-7 and NCI-H460 cell lines. This study highlights the potential of O. quadripartita fractions rich in phenolic compounds to be used in pharmaceutical and cosmetic fields.