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Advisor(s)
Abstract(s)
The freq uency and severity of diagnosed fungal infections is growing and, linked with
thi s, it is observed an exacerbated increase of the antifungal drugs resistance. Candida
albicans has been considered the main agent responsible for opportunistic pathogenic
infections, but, recently, other non-C. albicans Candida species have also been considered
[1]. In face to the increased number of Candida species drug resistant, it is crucial
to use natural matrices to discover efficient alternative therapies to current antifungal
agents. Salvia officina /is L., commonly known as sage, could be one of those natural matrices,
containing a wide variety of bioactive molecules. Despite the existence of some
reports on antimicrobial activity of sage, the most stud ied preparation is the essential
oil, being scarce the aqueous extracts. The antifungal activity of decoction, infusion and
hydroalcoholic extract of cultivated sage were evaluated, and further characterized in
terms of phenolic composition (HPLC- DAD- ESIIMS). Antifungal activity was assessed
against a total of 19 Candida strains of C. albicans, C. tropicalis, C. parapsilosis and
C. glabrata recovered from different human biological samples. The most pronounced
effect was observed against C. parapsilosis, fo llowed by C. tropicalis and C. albicans. No
effect was observed against the three C. glabrata stra ins. In general, decoction presented
the most pronounced effect, followed by infusion and hydroalcoholic extract, varying
directly according with the extract concentration. Decoction also showed the highest
concentration in phenolic compounds, followed by hydroalcoholic extract and infusions.
Rosmarinic acid and luteolin 7-0-glucuronide were the main phenolic compounds. Thus,
the use of sage decoction, incorporated on daily food dietary, or even as complement to
an antifungal therapy, could provide considerable benefits, not only by internal but also
external use, without the common side effects reported for essential oil.
Description
Keywords
Antifungal activity Candida species Salvia officina/is L. Phenolic compounds
Citation
Martins, Natália; Barros, Lillian; Silva, Sónia; Santos-Buelga, Celestino; Henriques, Mariana; Ferreira, Isabel C.F.R. (2014). Antifungal activity against Candida species and phenolic characterization of decoction, infusion and hydroalcoholic extract of cultivated Salvia officinalis L. In 62th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research - GA2014. Guimarães