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Advisor(s)
Abstract(s)
Cryphonectria parasitica, the causal agent of chestnut blight, causes necrotic
lesions (so-called cankers) on the bark of stems and branches of susceptible host trees.
Cryphonectria Hypovirus 1 (CHV1) infects C. parasitica and reduces the fungus
virulence (hypovirulence) and alters the fungus morphology in culture (pigmentation and
sporulation capacity). By this characteristics the mycovirus CHV1 is used in Europe as a
biological control agent of Chestnut Blight.The aim of this project is to better understand
the effect of the mycovirus on the fungi pathogenicity by comparing the production of
some lignin degrading enzymes and the metabolic profiles of some virulent and
hypovirulent (converted and original) strains. For qualitative evaluation, several different
compounds have been used as indicators for ligninolytic enzymes production. For
quantitative evaluation, among 9 strains 5 were chosen for biological tests and cultivation
in minimal liquid media and the amount of enzyme produced were analysed. Virulent
strains were found to cause more damage in chestnut branches and to produce more lignin
degrading enzymes. In apple fruits, some CHV1 strains produced bigger rot lesions than
wild type strains did. In parallel, Biolog FF MicroPlates have been used for the first time
with Cryphonectria parasitica to assess their metabolic profiles by the utilization of 95
different carbon sources. Carbohydrates, amino acids, amines/amides, miscellaneous and
polymers were found to be more consumed by hypovirulent strains; therefore, this may
suggest. an adaptation in this fungal strains ecology and field fitness.
Description
Keywords
Cryphonectria parasitica Biolog FF MicroPlates Ligninolytic enzymes Cryphonectria Hypovirus 1 (CHV1)
Citation
Abdellaziz, Omar; Jorge, Lurdes; Moura, Luísa; Coelho, Valentim; Gouveia, Maria Eugénia (2019). Characterization of ligninolytic enzymes and metabolic profile of cryphonectria parasitica and the isogenic converted strains by CHV1 hypovirus. In III Congresso Nacional das Escolas Superiores Agrárias (IIICNESA). Viseu
Publisher
Instituto Politécnico de Viseu