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Characterization of ligninolytic enzymes and metabolic profile of cryphonectria parasitica and the isogenic converted strains by CHV1 hypovirus

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dc.contributor.authorAbdellaziz, Omar
dc.contributor.authorJorge, Lurdes
dc.contributor.authorMoura, Luísa
dc.contributor.authorCoelho, Valentim
dc.contributor.authorGouveia, Maria Eugénia
dc.date.accessioned2020-03-19T09:58:10Z
dc.date.available2020-03-19T09:58:10Z
dc.date.issued2019
dc.description.abstractCryphonectria parasitica, the causal agent of chestnut blight, causes necrotic lesions (so-called cankers) on the bark of stems and branches of susceptible host trees. Cryphonectria Hypovirus 1 (CHV1) infects C. parasitica and reduces the fungus virulence (hypovirulence) and alters the fungus morphology in culture (pigmentation and sporulation capacity). By this characteristics the mycovirus CHV1 is used in Europe as a biological control agent of Chestnut Blight.The aim of this project is to better understand the effect of the mycovirus on the fungi pathogenicity by comparing the production of some lignin degrading enzymes and the metabolic profiles of some virulent and hypovirulent (converted and original) strains. For qualitative evaluation, several different compounds have been used as indicators for ligninolytic enzymes production. For quantitative evaluation, among 9 strains 5 were chosen for biological tests and cultivation in minimal liquid media and the amount of enzyme produced were analysed. Virulent strains were found to cause more damage in chestnut branches and to produce more lignin degrading enzymes. In apple fruits, some CHV1 strains produced bigger rot lesions than wild type strains did. In parallel, Biolog FF MicroPlates have been used for the first time with Cryphonectria parasitica to assess their metabolic profiles by the utilization of 95 different carbon sources. Carbohydrates, amino acids, amines/amides, miscellaneous and polymers were found to be more consumed by hypovirulent strains; therefore, this may suggest. an adaptation in this fungal strains ecology and field fitness.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAbdellaziz, Omar; Jorge, Lurdes; Moura, Luísa; Coelho, Valentim; Gouveia, Maria Eugénia (2019). Characterization of ligninolytic enzymes and metabolic profile of cryphonectria parasitica and the isogenic converted strains by CHV1 hypovirus. In III Congresso Nacional das Escolas Superiores Agrárias (IIICNESA). Viseupt_PT
dc.identifier.urihttp://hdl.handle.net/10198/21053
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherInstituto Politécnico de Viseupt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCryphonectria parasiticapt_PT
dc.subjectBiolog FF MicroPlatespt_PT
dc.subjectLigninolytic enzymespt_PT
dc.subjectCryphonectria Hypovirus 1 (CHV1)pt_PT
dc.titleCharacterization of ligninolytic enzymes and metabolic profile of cryphonectria parasitica and the isogenic converted strains by CHV1 hypoviruspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceViseupt_PT
oaire.citation.endPage89pt_PT
oaire.citation.startPage89pt_PT
oaire.citation.titleIII Congresso Nacional das Escolas Superiores Agrárias (IIICNESA)pt_PT
person.familyNameCoelho
person.familyNameGouveia
person.givenNameValentim
person.givenNameMaria Eugénia
person.identifier.ciencia-id0618-49BC-EACE
person.identifier.orcid0000-0001-5829-7669
person.identifier.orcid0000-0002-2550-9108
person.identifier.scopus-author-id26533798200
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication99a97d58-8b4c-4a49-9855-d334d568b9f3
relation.isAuthorOfPublication99e4701b-e3c2-4594-a37f-42bd49821d6e
relation.isAuthorOfPublication.latestForDiscovery99e4701b-e3c2-4594-a37f-42bd49821d6e

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