Advisor(s)
Abstract(s)
Although cholesterol is essential for the functioning of the human body, its excess is responsible for
causing atherosclerosis, along with several other diseases that affect the bones, liver, hormones, and
immune system1. As a natural solution, mushrooms are being studied for their ability to reduce
cholesterol due to their richness in molecules capable of reducing both cholesterol absorption and
synthesis2,3. Therefore, the present work investigated two mushrooms species (Agaricus bisporus L.
and Pleurotus ostreatus L.), aiming at extracting the highest contents of statins and ergosterol.
Ultrasound assisted extraction (UAE) optimization was performed followed by quantification of
ergosterol and statins of the extracts by HPLC-UV4,5, as well as the effect of the temperature on both
extractions (with and without iced bath), since statins are described as sensitive to high temperatures.
For A. bisporus, there was no significant differences in the concentration of ergosterol and pravastatin
when applying ice bath during the UAE. For P. ostreatus, the ice bath resulted in a 6% decrease in the
ergosterol concentration and a 35% increase in pravastatin content, suggesting that the best extraction
method to obtain an extract rich in hypocholesterolemic compounds is a cold extraction. For
optimization, the P. ostreatus extract revealed the highest amount of pravastatin and ergosterol (63.85
and 23.95 mg/g of extract, respectively), in which the best extraction conditions were 400 W of
ultrasonic power, 4 minutes and 44 seconds of extraction time, and 15.2 g/L of solid:liquid ratio, using
methanol as solvent, besides using an ultrasound extraction with ice bath.
Description
Keywords
Ergosterol Mushroom Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology
Pedagogical Context
Citation
Ueda, Jonata Massao; Benhassen, Yosra; Carocho, Márcio; Barros, Lillian; Calhelha, Ricardo C.; Heleno, Sandrina A. (2022). Development of statin-like and ergosterol enriched extracts from mushrooms bioresidues. In Livro de Resumos do 12º Encontro Nacional de Cromatografia & XIV WARPA. Aveiro
Publisher
Sociedade Portuguesa de Química