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Advisor(s)
Abstract(s)
The sonoextraction (SE) of aescin saponins and phenolic compounds from the inedible seed kernels of Aesculus
hippocastanum was optimized using a central composite rotatable design coupled with response surface methodology,
where the joint effects of ultrasonic power, sonication time, and ethanol proportion were investigated.
Flavonol glycosides (≥90 % of the phenolic fraction), flavan-3-ols, phenolic acids, and aescin saponins were
identified by HPLC-DAD-ESI/MSn, and the quantitative data was fitted to a quadratic model to predict the
optimal SE conditions. After validating the models, the significant effect of the three factors was confirmed. The
extraction of flavonols was maximized to 48 ± 2 mg/g extract by SE at 105.9 W for 4.1 min in 83.9 % ethanol,
while 19.9 min sonication at 100.9 W in 95.8 % ethanol favored the recovery of 3.8 ± 0.1 mg/g extract of aescin
saponins. A process for simultaneous SE of both classes of phytochemicals was also established. Overall, these SE
processes proved to be time-saving and selective for the A. hippocastanum seed active constituents, which are
cognized for their wide range of bioactivities and applications in the pharmaceutical, cosmetic, and food
industries.
Description
Keywords
Horse chestnut seed extracts Sonoextraction Aescin saponins Flavonol glycosides Chromatographic analysis Extraction optimization
Pedagogical Context
Citation
Publisher
Elsevier