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Advisor(s)
Abstract(s)
Ganoderma lucidum is a well-known medicinal mushroom, both historically and currently. Driven by
the ethnopharmacological prospect and the crescent body of scientific evidence that associates G.
lucidum intake with health, the interest in its secondary metabolites has been further fostered.
Whereas most research on medicinal mushrooms has focused on the comprehensive identification
and yields of metabolites throughout their different growth phases, the distribution of those
compounds along the sporocarps (fruiting bodies) in the
poorly investigated. This study aimed to directly compare the chemical composition of the exterior
skin and interior flesh of G. lucidum sporocarps. Dried samples provided by Käapa Biotech (Finland)
were homogenised and subjected to ultrasound-assisted extraction with ethanol:H2O (80:20, v/v) at
an amplitude of 47% for 15 min. Extracts were analysed in an HPLC-DAD-(ESI-)MS/MS system. Sixtytwo
compounds were tentatively identified in both extracts and comprised primarily lanostane-type
triterpenes, besides six phenolic compounds. Among the triterpenes, 20 lucidenic and 16 ganoderic
acids were found, with Lucidenic acid F and Ganoderic acid D being the major compounds in the flesh
and skin. The overall chemical profile was the same regardless of the sporocarp part analysed, but the
proportion among the compounds was considerably different. Interestingly, whereas the total
triterpene content of the outer part exceeded 3 times that of the inner part, both presented equivalent
amounts of total polyphenols. Our results indicate a similar profile but a higher concentration of
compounds in the skin when compared to the interior biomass of fruiting bodies. This is the first time
a study has examined the variations of triterpenic components between different parts of G. lucidum
sporocarps.
Description
Keywords
Ganoderma lucidum sporocarps Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology
Citation
Rodrigues, Daniele B.; Oludemi, Taofiq; Petros, Peter; Barros, Lillian (2022). Comprehensive HPLC-DAD-(ESI-)MS/MS characterization and comparison of bioactive molecules from different parts of Ganoderma lucidum sporocarps. In Livro de Resumos do 12º Encontro Nacional de Cromatografia & XIV WARPA. Aveiro
Publisher
Sociedade Portuguesa de Química