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Comprehensive HPLC-DAD-(ESI-)MS/MS characterization and comparison of bioactive molecules from different parts of Ganoderma lucidum sporocarps

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dc.contributor.authorRodrigues, Daniele Bobrowski
dc.contributor.authorOludemi, Taofiq
dc.contributor.authorPetros, Peter
dc.contributor.authorBarros, Lillian
dc.date.accessioned2023-03-17T09:48:28Z
dc.date.available2023-03-17T09:48:28Z
dc.date.issued2022
dc.description.abstractGanoderma lucidum is a well-known medicinal mushroom, both historically and currently. Driven by the ethnopharmacological prospect and the crescent body of scientific evidence that associates G. lucidum intake with health, the interest in its secondary metabolites has been further fostered. Whereas most research on medicinal mushrooms has focused on the comprehensive identification and yields of metabolites throughout their different growth phases, the distribution of those compounds along the sporocarps (fruiting bodies) in the poorly investigated. This study aimed to directly compare the chemical composition of the exterior skin and interior flesh of G. lucidum sporocarps. Dried samples provided by Käapa Biotech (Finland) were homogenised and subjected to ultrasound-assisted extraction with ethanol:H2O (80:20, v/v) at an amplitude of 47% for 15 min. Extracts were analysed in an HPLC-DAD-(ESI-)MS/MS system. Sixtytwo compounds were tentatively identified in both extracts and comprised primarily lanostane-type triterpenes, besides six phenolic compounds. Among the triterpenes, 20 lucidenic and 16 ganoderic acids were found, with Lucidenic acid F and Ganoderic acid D being the major compounds in the flesh and skin. The overall chemical profile was the same regardless of the sporocarp part analysed, but the proportion among the compounds was considerably different. Interestingly, whereas the total triterpene content of the outer part exceeded 3 times that of the inner part, both presented equivalent amounts of total polyphenols. Our results indicate a similar profile but a higher concentration of compounds in the skin when compared to the interior biomass of fruiting bodies. This is the first time a study has examined the variations of triterpenic components between different parts of G. lucidum sporocarps.pt_PT
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES (PIDDAC) to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2021). L.B. also thanks FCT through the institutional scientific employment program contract for her contract. D.B.R. would like to acknowledge the Fundo Europeu de Desenvolvimento Regional -01-0247-FEDER- 113508). MICINN also supports the Juan de la Cierva Formation contract for T. Oludemi (FJC2019-042549-I).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRodrigues, Daniele B.; Oludemi, Taofiq; Petros, Peter; Barros, Lillian (2022). Comprehensive HPLC-DAD-(ESI-)MS/MS characterization and comparison of bioactive molecules from different parts of Ganoderma lucidum sporocarps. In Livro de Resumos do 12º Encontro Nacional de Cromatografia & XIV WARPA. Aveiropt_PT
dc.identifier.isbn978-989-8124-37-1
dc.identifier.urihttp://hdl.handle.net/10198/27801
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSociedade Portuguesa de Químicapt_PT
dc.relationLA/P/0007/2021pt_PT
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectGanoderma lucidum sporocarpspt_PT
dc.subjectResearch Subject Categories::TECHNOLOGY::Chemical engineering::Food technologypt_PT
dc.titleComprehensive HPLC-DAD-(ESI-)MS/MS characterization and comparison of bioactive molecules from different parts of Ganoderma lucidum sporocarpspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.citation.conferencePlaceAveiropt_PT
oaire.citation.endPage197pt_PT
oaire.citation.startPage197pt_PT
oaire.citation.titleLivro de Resumos do 12º Encontro Nacional de Cromatografia & XIV WARPApt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameRodrigues
person.familyNameOludemi
person.familyNameBarros
person.givenNameDaniele Bobrowski
person.givenNameTaofiq
person.givenNameLillian
person.identifier469085
person.identifier.ciencia-idA91B-FE1D-B275
person.identifier.ciencia-idE318-A5CD-6626
person.identifier.ciencia-id9616-35CB-D001
person.identifier.orcid0000-0003-0260-5751
person.identifier.orcid0000-0003-2822-3532
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id35236343600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication18a661e9-0f77-4bd1-b2b0-5b929b760ab3
relation.isAuthorOfPublication74ac0f55-9896-40ec-8444-523044e80c4a
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