Advisor(s)
Abstract(s)
Fungi are known to modify the properties of lignocellulosic materials during solid-state fermentation (SSF). In
this study, agricultural side-streams (sunflower seed hulls, rice husks and rice straw) were used as substrates for
SSF with dikaryotic and monokaryotic strains of Pleurotus sapidus. The phenolic profiles of the mentioned
substrates were characterized by LC-DAD/ESI-MSn pre- and post- fermentation. Moreover, antioxidant, cytotoxic
and antimicrobial activities were screened against oxidizable cellular substrates, tumour and primary cell lines,
and different bacteria and fungi, respectively. The concentration of phenolic compounds in the crop side-streams
was reduced after fermentation with both strains of the fungus. The fermented extracts also displayed lower
antioxidant and cytotoxic activities and had no hepatotoxicity. The antimicrobial activity depended upon the
crop side-stream and/or SSF conditions. These results indicate that P. sapidus represent a good candidate to
modify the phenolic fraction presents in crop side-streams with a consequent decrease in its bioactivities.
However, the SSF with P. sapidus strains play an interesting role in the detoxification of plant materials which
can be used for different applications according to the “reduce - reuse - recycle” concept contributing with the
sustainable land use and circular economy.
Description
Keywords
Antimicrobial activity Antioxidant activity Lignocellulosic substrates Phenolic compounds Pleurotus sapidus Solid-state fermentation
Pedagogical Context
Citation
Pinela, José; Omarini, Alejandra B.; Stojković, Dejan; Barros, Lillian; Postemsky, Pablo D.; Calhelha, Ricardo C.; Breccia, Javier; Fernández-Lahore, Marcelo; Soković, Marina; Ferreira, Isabel C.F.R. (2020). Biotransformation of rice and sunflower side-streams by dikaryotic and monokaryotic strains of Pleurotus sapidus: Impact on phenolic profiles and bioactive properties. Food Research International. ISSN 0963-9969. 132, p. 1-10