Advisor(s)
Abstract(s)
The infusions of Thymus pallescens Noë, Saccocalyx satureioides Coss. et Dur., Ptychotis verticillata Briq.
and Limoniastrum guyonianum Boiss. have been used as medicinal remedies for many diseases in Algerian
folk medicine. These species have also been well documented as rich sources of phytochemicals, such as
phenolic compounds with wide diversified chemical structures, which exhibit far-ranging biological activities.
Thus, the phenolic compound profile of the aqueous extracts, obtained by infusing, of the mentioned
species was obtained by HPLC-DAD-ESI/MS, and their antibacterial activity was evaluated against clinical
isolates. Several phenolic acids were identified and quantified, particularly caffeic acid derivatives along with
glycosylated flavonoids. T. pallescens and S. satureioides contain 13 phenolic compounds, where rosmarinic
acid was the most abundant phenolic acid present, while L. guyonianum presented myricetin-3-O-glucoside
and myricetin-O-rhamnoside as the main compounds among the eight detected molecules.
P. verticillata presented a profile of ten phenolic compounds, where 5-O-caffeoylquinic acid was the most
abundant phenolic acid, followed by the flavone luteolin-3-O-glucoside. The antibacterial activity of the
infusions ranged between 2.5 and 20 mg mL−1 (MIC values), and L. guyonianum showed the highest activity
against all of the tested bacteria, Staphylococcus aureus and Pseudomonas aeruginosa being the most
sensitive and resistant strains, respectively. Thus, the studied plant species are sources of natural antibacterial
substances that can be used to fight against pathogenic microorganisms.
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Pedagogical Context
Citation
Ziani, Borhane E.C.; Barros, Lillian; Boumehira, Ali Z.; Bachari, Khaldoun; Heleno, Sandrina A.; Alves, Maria Jose; Ferreira, Isabel C.F.R. (2018). Profiling polyphenol composition by HPLC-DAD-ESI/MSn and the antibacterial activity of infusion preparations obtained from four medicinal plants. Food and Function. ISSN 2042-6496. 9, p. 149-159