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Advisor(s)
Abstract(s)
Tripleurospermum inodorum (L.) Sch. Bip. (Asteraceae), scentless mayweed, is a common
weed species, but despite its abundancy it is still underexplored in the terms of its
bioactivities. The aim of the study was to explore the bioactivities of scentless mayweed
methanolic extract including antimicrobial, antibiofilm, antioxidant, cytotoxic, and antiinflammatory
activity and to provide deeper insight into its chemical composition. It was
shown that T. inodorum inhibits growth of human pathogenic microorganisms with minimal
inhibitory concentration (MIC) in range 3-12 mg/mL, with the lowest MIC noted for the
species Candida albicans 475/15, C. albicans 17/15, C. parapsilosis ATCC 22019,
Pseudomonas aeruginosa (IBRS P001), Yersinia enterocolitica (ATCC 23715), and
Klebsiella pneumoniae (ATCC 13883). Its biofilm potential was promising - application of
plant extract (1.5 and 3 mg/mL) reduced C. albicans 475/15 biofilm biomass and biofilm cell
viability for more than 50%. Antioxidant assays suggested the plants antioxidant potential at
442.2 mmol GAE (gallic acid equivalent)/100 mg DW (dry weight) as in ABTS+ assay,
277.8 mmol GAE/100 mg DW in DPPH∙, and 204.4 mmol GAE/100 mg DW as recorded in
the FRAP assay. Wide cytotoxic potential of the extract was noticed for the range of cells:
AGS (gastric adenocarcinoma, GI50 198.7 μg/mL), CaCo2 (colorectal adenocarcinoma, GI50
278.2 μg/mL), MCF-7 (breast adenocarcinoma, GI50 265.7 μg/mL), NCI-H460 (lung
carcinoma, GI50 62.9 μg/mL), and VERO (non-tumour cell line, GI50 >400 μg/mL). Likewise,
low IC50 (8.4 μg/mL) in the RAW 246.7 anti-inflammatory assay suggests the strong antiinflammatory
potential of this weed. The dominant polyphenols in the extract were two
apigenin derivatives: apigenin-O-pentoside and apigenin-O-acetylhexoside (5.234 mg/g and
4.929 mg/g extract, respectively). T. inodorum is a rich source of different bioactive
polyphenols and its methanolic extract displays a range of bioactive features that should be
further examined in order to develop novel herbal medicines
Description
Keywords
Tripleurospermum inodorum Research Subject Categories::FORESTRY, AGRICULTURAL SCIENCES and LANDSCAPE PLANNING::Product science
Pedagogical Context
Citation
Ivanov, Marija; Božunović, Jelena; Dias, Maria Inês; Calhelha, Ricardo C.; Barros, Lillian; Ferreira, Isabel C.F.R.; Stojković, Dejan (2022). Tripleurospermum inodorum – mayweed without scent but with range of bioactivities. In 1st European Symposium on Phytochemicals in Medicine and Food.1-EuSPMF: Book of abstracts. Sérvia
Publisher
University of Belgrade