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Advisor(s)
Abstract(s)
Aesculus hippocastanum L. (Sapindaceae) is a large, broad-crowned deciduous tree cultivated
as an ornamental in urban areas in the Northern hemisphere. Among the scientific community,
it is known mainly for steroidal glycosides, among which aescin is the main active constituent
and responsible for most of its medicinal properties [1,2]; but other bioactive compounds such
as flavonol glycosides have also been described in this plant [3]. In folk medicine, while the
bark has been used to treat dermatitis, the leaves and seeds are used for their antiinflammatory
effect [2]. In Europe, seed extracts and supplements have been used to treat
varicose veins among other venous disorders [4]. Therefore, this study aimed to characterize
the phenolic and saponin profiles of the kernel, shell, and husk of the horse chestnut fruit.
After separating, lyophilizing, and grinding the three fruit parts, hydroethanolic extracts were
prepared by sonoextraction at 400 W (20 kHz) for 40 min. The chromatographic separation
and identification of phenolic compounds and saponins were achieved by high-performance
liquid chromatography coupled with photodiode array detection and electrospray ionization
tandem mass spectrometry (HPLC-DAD-ESI/MS), using different elution gradients [5]. It
was possible to identify 31 phenolic compounds in the hydroethanolic extracts, including
phenolic acids (caffeic acid and p-coumaroyl derivatives), flavan-3-ols ((+)-catechin and (-)-
epicatechin derivatives), and flavonols (O-glycosylated quercetin, isorhamnetin derivatives,
and kaempferol). While flavonols and flavan-3-ols predominated in the seed kernel and shell,
respectively, the husk had comparable amounts of both phenolic constituents. The four main
aescin saponins were consistently detected in the seed kernel extracts, namely aescin Ia and Ib
(β-aescin) and isoaescin Ia and Ib (α-aescin). Overall, the different parts of the inedible horse
chestnut fruit have a different phytochemical profile in qualitative and quantitative terms.
Further studies will be important to evaluate biological activities of the different seed extracts
Description
Keywords
Aesculus hippocastanum L. Research Subject Categories::FORESTRY, AGRICULTURAL SCIENCES and LANDSCAPE PLANNING::Product science
Pedagogical Context
Citation
Dias, Maria Inês; Pereira, Carla; Albiston, Carly; Pinela, José; Barros, Lillian (2022). HPLC-DAD-ESI/MS profiling of phytochemicals in Aesculus hippocastanum fruit. In 1st European Symposium on Phytochemicals in Medicine and Food.1-EuSPMF: Book of abstracts. Sérvia
Publisher
University of Belgrade