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HPLC-DAD-ESI/MS profiling of phytochemicals in Aesculus hippocastanum fruit

2022Conference object Open access
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dc.contributor.authorDias, Maria Inês
dc.contributor.authorPereira, Carla
dc.contributor.authorAlbiston, Carly
dc.contributor.authorPinela, José
dc.contributor.authorBarros, Lillian
dc.date.accessioned2023-01-06T09:51:35Z
dc.date.available2023-01-06T09:51:35Z
dc.date.issued2022
dc.description.abstractAesculus hippocastanum L. (Sapindaceae) is a large, broad-crowned deciduous tree cultivated as an ornamental in urban areas in the Northern hemisphere. Among the scientific community, it is known mainly for steroidal glycosides, among which aescin is the main active constituent and responsible for most of its medicinal properties [1,2]; but other bioactive compounds such as flavonol glycosides have also been described in this plant [3]. In folk medicine, while the bark has been used to treat dermatitis, the leaves and seeds are used for their antiinflammatory effect [2]. In Europe, seed extracts and supplements have been used to treat varicose veins among other venous disorders [4]. Therefore, this study aimed to characterize the phenolic and saponin profiles of the kernel, shell, and husk of the horse chestnut fruit. After separating, lyophilizing, and grinding the three fruit parts, hydroethanolic extracts were prepared by sonoextraction at 400 W (20 kHz) for 40 min. The chromatographic separation and identification of phenolic compounds and saponins were achieved by high-performance liquid chromatography coupled with photodiode array detection and electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI/MS), using different elution gradients [5]. It was possible to identify 31 phenolic compounds in the hydroethanolic extracts, including phenolic acids (caffeic acid and p-coumaroyl derivatives), flavan-3-ols ((+)-catechin and (-)- epicatechin derivatives), and flavonols (O-glycosylated quercetin, isorhamnetin derivatives, and kaempferol). While flavonols and flavan-3-ols predominated in the seed kernel and shell, respectively, the husk had comparable amounts of both phenolic constituents. The four main aescin saponins were consistently detected in the seed kernel extracts, namely aescin Ia and Ib (β-aescin) and isoaescin Ia and Ib (α-aescin). Overall, the different parts of the inedible horse chestnut fruit have a different phytochemical profile in qualitative and quantitative terms. Further studies will be important to evaluate biological activities of the different seed extractspt_PT
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES (PIDDAC) to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2021). To FCT for the contract of M.I. Dias (CEEC Institucional), C. Pereira (CEEC Institucional), J. Pinela (CEECIND/01011/2018), and L. Barros (CEEC Institucional).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDias, Maria Inês; Pereira, Carla; Albiston, Carly; Pinela, José; Barros, Lillian (2022). HPLC-DAD-ESI/MS profiling of phytochemicals in Aesculus hippocastanum fruit. In 1st European Symposium on Phytochemicals in Medicine and Food.1-EuSPMF: Book of abstracts. Sérviapt_PT
dc.identifier.isbn978‐86‐7834‐408‐4
dc.identifier.urihttp://hdl.handle.net/10198/26352
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherUniversity of Belgradept_PT
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.relationNot Available
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAesculus hippocastanum L.pt_PT
dc.subjectResearch Subject Categories::FORESTRY, AGRICULTURAL SCIENCES and LANDSCAPE PLANNING::Product sciencept_PT
dc.titleHPLC-DAD-ESI/MS profiling of phytochemicals in Aesculus hippocastanum fruitpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2018/CEECIND%2F01011%2F2018%2FCP1578%2FCT0002/PT
oaire.citation.conferencePlaceSérviapt_PT
oaire.citation.endPage41pt_PT
oaire.citation.startPage41pt_PT
oaire.citation.title1st European Symposium on Phytochemicals in Medicine and Food.1-EuSPMFpt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamCEEC IND 2018
person.familyNameDias
person.familyNamePereira
person.familyNamePinela
person.familyNameBarros
person.givenNameMaria Inês
person.givenNameCarla
person.givenNameJosé
person.givenNameLillian
person.identifier1415151
person.identifier469085
person.identifier.ciencia-id2A13-4BE6-C7CF
person.identifier.ciencia-idEF10-2739-2B70
person.identifier.ciencia-id771C-2B43-B108
person.identifier.ciencia-id9616-35CB-D001
person.identifier.orcid0000-0001-8744-7814
person.identifier.orcid0000-0003-0093-771X
person.identifier.orcid0000-0001-7523-1637
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridM-8242-2013
person.identifier.ridK-1629-2016
person.identifier.ridB-4466-2014
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id54388787000
person.identifier.scopus-author-id55627876424
person.identifier.scopus-author-id54392272800
person.identifier.scopus-author-id35236343600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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