Low-cost alternative for the bioproduction of bioactive phenolic compounds of callus cultures from Cereus hildmannianus (K.) Schum

The aim of this study was to establish a sustainable alternative callus culture of Cereus hildmannianus for the production and bioactive determination of phenolic compounds from this species. The conventional callus was cultivated using agar and Murashige and Skoog (MS) medium, while for the alternative culture the agar was replaced with a cotton support covered with filter paper and MS medium (incubated at 32 ◦C with photoperiod of 16 h), and the morphological characteristics and growth index were assessed (8 weeks). Extracts were obtained by maceration followed by partition, characterized by nuclear magnetic resonance - NMR and ultra-high performance liquid chromatography - UHPLC, quantified (phenolic compounds) by UV-Vis methods, and their antioxidant, antitumor activities, as well as cytotoxicity, were evaluated. The establishment of an alternative callus culture was carried out successfully. Characteristic signals of phenolic compounds were determined by NMR, and 46 compounds with fragment ions were identified using UHPLC analysis. The highest concentrations of phenolic compounds, and greatest antioxidant and antitumor activities, were obtained with the dichloromethane fractions of both callus tissue cultures, which were not cytotoxic. The callus culture from C. hildmannianus has shown promise as a source for the sustainable production of phenolic compounds with antioxidant and antiproliferative activities and thus, has potential use as a natural antitumor product.

Keywords

Alternative culture Cactus Mass spectrometry Medicinal herbs Phytochemicals

URI

http://hdl.handle.net/10198/24602

Citation

Santos, Everton da Silva; Savam, Aline; Cabral, Marcia Regina Pereira; Castro, Juliana Cristina; Collet, Sandra Aparecida de Oliveira; Mandim, Filipa; Calhelha, Ricardo C.; Barros, Lillian; Machado, Maria de Fatima Pires da Silva; de Oliveira, Arildo Jose Braz; Goncalves, Regina Aparecida Correia. (2022). Low-cost alternative for the bioproduction of bioactive phenolic compounds of callus cultures from Cereus hildmannianus (K.) Schum. Journal of Biotechnology. EISSN 1873-4863. 356, p. 8-18