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Low-cost alternative for the bioproduction of bioactive phenolic compounds of callus cultures from Cereus hildmannianus (K.) Schum

2022Artigo científico Acesso aberto
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dc.contributor.authorSantos, Everton da Silva
dc.contributor.authorSavam, Aline
dc.contributor.authorCabral, Marcia Regina Pereira
dc.contributor.authorCastro, Juliana Cristina
dc.contributor.authorCollet, Sandra Aparecida de Oliveira
dc.contributor.authorMandim, Filipa
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorBarros, Lillian
dc.contributor.authorMachado, Maria de Fatima Pires da Silva
dc.contributor.authorOliveira, Arildo Jose Braz
dc.contributor.authorGonçalves, Regina Aparecida Correia
dc.date.accessioned2022-01-12T16:44:32Z
dc.date.available2022-01-12T16:44:32Z
dc.date.issued2022
dc.description.abstractThe aim of this study was to establish a sustainable alternative callus culture of Cereus hildmannianus for the production and bioactive determination of phenolic compounds from this species. The conventional callus was cultivated using agar and Murashige and Skoog (MS) medium, while for the alternative culture the agar was replaced with a cotton support covered with filter paper and MS medium (incubated at 32 ◦C with photoperiod of 16 h), and the morphological characteristics and growth index were assessed (8 weeks). Extracts were obtained by maceration followed by partition, characterized by nuclear magnetic resonance - NMR and ultra-high performance liquid chromatography - UHPLC, quantified (phenolic compounds) by UV-Vis methods, and their antioxidant, antitumor activities, as well as cytotoxicity, were evaluated. The establishment of an alternative callus culture was carried out successfully. Characteristic signals of phenolic compounds were determined by NMR, and 46 compounds with fragment ions were identified using UHPLC analysis. The highest concentrations of phenolic compounds, and greatest antioxidant and antitumor activities, were obtained with the dichloromethane fractions of both callus tissue cultures, which were not cytotoxic. The callus culture from C. hildmannianus has shown promise as a source for the sustainable production of phenolic compounds with antioxidant and antiproliferative activities and thus, has potential use as a natural antitumor product.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSantos, Everton da Silva; Savam, Aline; Cabral, Marcia Regina Pereira; Castro, Juliana Cristina; Collet, Sandra Aparecida de Oliveira; Mandim, Filipa; Calhelha, Ricardo C.; Barros, Lillian; Machado, Maria de Fatima Pires da Silva; de Oliveira, Arildo Jose Braz; Goncalves, Regina Aparecida Correia. (2022). Low-cost alternative for the bioproduction of bioactive phenolic compounds of callus cultures from Cereus hildmannianus (K.) Schum. Journal of Biotechnology. EISSN 1873-4863. 356, p. 8-18pt_PT
dc.identifier.issn1873-4863
dc.identifier.urihttp://hdl.handle.net/10198/24602
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherELSEVIER
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAlternative culturept_PT
dc.subjectCactuspt_PT
dc.subjectMass spectrometrypt_PT
dc.subjectMedicinal herbspt_PT
dc.subjectPhytochemicals
dc.titleLow-cost alternative for the bioproduction of bioactive phenolic compounds of callus cultures from Cereus hildmannianus (K.) Schumpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleJournal of Biotechnologypt_PT
person.familyNameMandim
person.familyNameCalhelha
person.familyNameBarros
person.givenNameFilipa
person.givenNameRicardo C.
person.givenNameLillian
person.identifier469085
person.identifier.ciencia-idF318-9049-EB99
person.identifier.ciencia-idF313-E3CE-554E
person.identifier.ciencia-id9616-35CB-D001
person.identifier.orcid0000-0002-6494-2326
person.identifier.orcid0000-0002-6801-4578
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridJ-2172-2014
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id6507978333
person.identifier.scopus-author-id35236343600
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationbcc274d4-7e6c-46a8-8e49-2752f9dbfbcd
relation.isAuthorOfPublication2d5d1a41-7561-4a01-871c-b4c97da35053
relation.isAuthorOfPublication3af07ffe-f914-48ba-a5d5-efcf70fdce01
relation.isAuthorOfPublication.latestForDiscovery2d5d1a41-7561-4a01-871c-b4c97da35053

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