Advisor(s)
Abstract(s)
The aim of this work was to produce an extract rich in different bioactive compounds from Arbutus unedo L. fruits
to enhance its possible valorization and commercialization. The conditions of the main variables of maceration,
microwave, and ultrasound assisted extractions (MA, MAE and UAE, respectively) were optimized and compared
in terms of its composition (based on the total material extracted, total content in carbohydrates, phenolic and
flavonoid compounds) and its preservative potential (based on the response of four in vitro antioxidant assays).
The key variables of each extraction technique (time, temperature or power and hydroalcoholic mixture) were
evaluated by specific experimental designs using response surface methodology. Mathematical models were
developed and numerical optimal values for each extraction technique and response were achieved. Regarding
the extraction of target compositional compounds, MAE was the most efficient, closely followed by MA. In terms
of its preservative potential, MAE was the most suitable solution, but MA gave similar results at lower temperatures
(~90 °C). Globally, MA and MAE were the best options conducting to optimal solutions using reduced
amounts of ethanol. UAE required higher ethanol contents (~60%). The results showed alternatives to obtain
extracts of A. unedo fruits, supporting their potential to be exploited at industrial level.
Description
Keywords
Antioxidant Arbutus unedo L. fruits Compositional analysis Conventional and non-conventional extraction techniques Maceration/microwave/ultrasound assisted extraction Response surface methodology
Pedagogical Context
Citation
Albuquerque, Bianca R.; Prieto, M. A.; Vazquez, J. A.; Barreiro, Maria Filomena; Barros, Lillian; Ferreira, Isabel C.F.R. (2018). Recovery of bioactive compounds from Arbutus unedo L. fruits: Comparative optimization study of maceration/microwave/ultrasound extraction techniques. Food Research International. ISSN 0963-9969. 109, p. 455-471