Percorrer por autor "Pinho, Diana"
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- 3D manufacturing of intracranial aneurysm biomodels for flow visualizations: low cost fabrication processesPublication . Souza, Andrews Victor Almeida; Souza, Mauren S.; Pinho, Diana; Agujetas, Rafael; Ferrera, Conrado; Lima, Rui A.; Puga, Hélder Fernandes; Ribeiro, J.E.There is a continuous search for better and more complete in vitro models with mechanical properties closer to in vivo conditions. In this work a manufacturing process, based on a lost core casting technique, is herein reported to produce aneurysm biomodels to perform experimental hemodynamic studies. By us- ing real artery images combined with a lost core casting technique, three materials were tested: paraffin, beeswax and glycerin-based soap. All in vitro biomodels were compared according to their transparency and final structure. Additionally, comparisons between experimental and numerical flow studies were also performed. The results have shown that the biomodels produced with beeswax and glycerine-based soap were the most suitable in vitro models to perform direct flow visualizations of particulate blood analogue fluids. The biomodels proposed in this works, have the potential to provide further insights into the complex blood flow phenomena happening at different kinds of pathologies and answer to important hemodynamics questions that otherwise cannot be tackled with the existing in vitro models.
- Accuracy/speed analysis of pipe friction factor correlationsPublication . Muzzo, Luiz Eduardo; Pinho, Diana; Lima, Luiz Eduardo Melo; Ribeiro, Luís FrölénThe Colebrook [1] equation is considered the standard for the calculation of friction factor for turbulent flow in commercial pipes, but it is implicit, and therefore it must be computed by iterative methods. Although such iterative computation quickly converges, the computational time in large pipe system simulations can be reduced using an accurate explicit correlation. A review of the up to date literature identified 30 different explicit correlations. In order to determine which correlation is the best alternative to Colebrook’s, both accuracy and computational burden were compared. The accuracy of each explicit correlation was compared against Colebrook’s correlation using the mean and maximum relative errors and the coefficient of determination. Also, the computational time of each equation was measured using the tic and toc functions in GNU Octave software. It was found that the iterative computation of the Colebrook equation demands about 2.6 times the computational time of the slowest explicit correlation. The correlations with the best balance between accuracy and computational burden are, in decreasing order of accuracy and increasing order of speed, correlations by Serghides [13] (Eqs. (17), (18), (19), and (20)), by Shacham [8] (Eqs. (10) and (11)), by Brkić and Praks [33] (Eqs. (53), (54), (55), and (56)), and by Fang et al. [19] (Eq. (28)).
- Advances in Microfluidic Systems and Numerical Modeling in Biomedical Applications: A ReviewPublication . Ferreira, Mariana; Carvalho, Violeta Meneses; Ribeiro, J.E.; Lima, Rui A.; Teixeira, Senhorinha F.C.F.; Pinho, DianaThe evolution in the biomedical engineering field boosts innovative technologies, with microfluidic systems standing out as transformative tools in disease diagnosis, treatment, and monitoring. Numerical simulation has emerged as a tool of increasing importance for better understanding and predicting fluid-flow behavior in microscale devices. This review explores fabrication techniques and common materials of microfluidic devices, focusing on soft lithography and additive manufacturing. Microfluidic systems applications, including nucleic acid amplification and protein synthesis, as well as point-of-care diagnostics, DNA analysis, cell cultures, and organ-on-a-chip models (e.g., lung-, brain-, liver-, and tumor-on-a-chip), are discussed. Recent studies have applied computational tools such as ANSYS Fluent 2024 software to numerically simulate the flow behavior. Outside of the study cases, this work reports fundamental aspects of microfluidic simulations, including fluid flow, mass transport, mixing, and diffusion, and highlights the emergent field of organ-on-a-chip simulations. Additionally, it takes into account the application of geometries to improve the mixing of samples, as well as surface wettability modification. In conclusion, the present review summarizes the most relevant contributions of microfluidic systems and their numerical modeling to biomedical engineering.
- An automatic method to track red blood cells in microchannelsPublication . Pinho, Diana; Gayubo, Fernando; Pereira, Ana I.; Lima, Rui A.Image analysis is extremely important to obtain crucial information about the blood phenomena in micro-circulation. The current study proposes an automatic method for segmentation and tracking red blood cells (RBCs) flowing through a 100 μm Glass capillary. The original images were obtained by means of a confocal system and then processed in Matlab using the Image Processing Toolbox. The automatic measurements ob-tained with the proposed automatic method are compared with a manual tracking method using a plugin from ImageJ.
- An in vitro experimental evaluation of the displacement field in an intracranial aneurysm modelPublication . Pinho, Diana; Bento, David; Ribeiro, J.E.; Lima, Rui A.; Vaz, M.A.P.The purpose of this paper is to develop a system able to study experimentally the displace-ment field of an in vitro intracranial aneurysm. Origin and growth of aneurysms is the result of a complex interaction between biological processes in the arterial wall and the involved hemo-dynamic phenomena’s. Once the aneurysm forms, the repetitive pressure and shear stresses exerted by the blood flow on the debilitated arterial wall can cause a gradual expansion. One promising method to evaluate and measure this expansion is to use optical field experimental techniques, such as interferometry. In this work the Electronic Speckle Pattern Interferometry was used to evaluate the deformation occurred on an intracranial aneurysm model fabricated in polydimensiloxane (PDMS) by using a 3D printer combined with a soft lithography tech-nique.
- Análise experimental e numérica de quedas de pressão em canais estenosadosPublication . Lopes, Sara Helena Carvalho; Fernandes, Carla S.; Pereira, Ana I.; Pinho, DianaO interesse nos estudos relacionados com o escoamento de fluidos em microcanais tem assumido um lugar de destaque na área de biomédica, uma vez que que estes estudos permitem analisar escoamentos sanguíneos evitando questões de ética que se colocam quando se trabalha fisicamente com o sangue. Numa primeira fase deste trabalho foram fabricados modelos anatómicos tridimensionais, mais concretamente canais circulares, um sem patologia e dois com ateromas de alturas diferentes, onde foram efetuadas medições de quedas de pressão. Com os resultados obtidos foi possível concluir que a presença de ateromas influencia o escoamento sanguíneo, verificando-se que as quedas de pressão aumentam com o aumento do grau da estenose. Numa segunda fase os escoamentos estudados experimentalmente foram alvo de um estudo numérico com o objetivo de comparar os resultados da simulação com os obtidos experimentalmente. As simulações numéricas foram efetuadas recorrendo a um software comercial que implementa o método dos volumes finitos e os resultados obtidos revelaram-se concordantes com os obtidos experimentalmente, o que permitiu concluir que a dinâmica de fluidos computacional é de facto uma boa estratégia a ser utilizada. Na fase final do trabalho, procedeu-se à identificação da melhor modelação matemática para caracterizar a diferença de valores obtidos nos estudos experimental e numérico. Após diversas estratégias conclui-se que a diferença de valores pode ser caracterizada por uma função polinomial.
- Análise experimental e numérica de quedas de pressão em canais estenosadosPublication . Lopes, Sara Helena Carvalho; Fernandes, Carla S.; Pereira, Ana I.; Pinho, DianaOs estudos relacionados com o escoamento de fluidos em microcanais têm sido alvo de várias investigações, tendo permitido prever que o escoamento sanguíneo é influenciado por várias patologias, como por exemplo, a aterosclerose. A aterosclerose consiste num estreitamento das artérias, devido a aglomerados de gordura que se acumulam no seu interior e que pode diminuir o abastecimento de sangue aos tecidos do organismo, nomeadamente órgãos vitais. Neste trabalho foi realizado uma análise experimental e numérica de quedas de pressão em canais estenosados. Os resultados experimentais e numéricos foram ainda otimizados recorrendo ao método dos mínimos quadrados por forma a encontrar uma expressão que traduza o problema em estudo. Por fim, com o presente trabalho pretende-se dar algum contributo na área do diagnóstico.
- Automatic tracking of labeled red blood cells in microchannelsPublication . Pinho, Diana; Lima, Rui A.; Pereira, Ana I.; Gayubo, FernandoThe current study proposes an automatic method for the segmentation and tracking of red blood cells flowing through a 100- m glass capillary. The original images were obtained by means of a confocal system and then processed in MATLAB using the Image Processing Toolbox. The measurements obtained with the proposed automatic method were compared with the results determined by a manual tracking method. The comparison was performed by using both linear regressions and Bland–Altman analysis. The results have shown a good agreement between the two methods. Therefore, the proposed automatic method is a powerful way to provide rapid and accurate measurements for in vitro blood experiments in microchannels.
- Blood flow in a bifurcation and confluence microchannel: effect of the cell-free layer in velocity profilesPublication . Pinho, Diana; Bento, David; Rodrigues, Raquel Oliveira; Fernandes, Carla S.; Garcia, Valdemar; Lima, Rui A.A few detailed studies have been performed in complex in vitro microvascular networks composed by bifurcations and confluences. The main purpose of the present work is to numerically simulate the flow of two distinct fluids through bifurcation and confluence geometries, i. e red blood cells (RBCs)suspended in Dextran40 with about 14% of heamatocrit and pure water. The simulations of pure water and RBCs flows were performed resorting to the commercial finite volume software package FLUENT. A well known hemodynamic phenomenon, known as Fahraeus-Lindqvist effect, observed in both in vivo and in vitro studies, results in the formation of a marginal cell-free layer (CFL) at regions adjacent to the wall. Recently, studies have shown that the formation of the CFL is affected by the geometry of the microchannel and for the case of the confluences a CFL tend to appear in the middle of the microchannel after the apex of the confluence. By using the CFL experimental data, the main objective of this work is to implement a CFL in the numerical simulations in order to obtain a better understanding of the effect of this layer on the velocity profiles.
- Blood flow in a bifurcation and confluence microchannel: the effect of the cell-free layer in the velocity profilesPublication . Pinho, Diana; Bento, David; Rodrigues, Raquel Oliveira; Fernandes, Carla S.; Garcia, Valdemar; Lima, Rui A.A few detailed studies have been performed in complex in vitro microvascular networks composed by bifurcations and confluences. The main purpose of the present work is to numerically simulate the flow of two distinct fluids through bifurcation and confluence geometries, i. e red blood cells (RBCs) suspended in Dextran40 with about 14% of heamatocrit and pure water. The simulations of pure water and RBCs flows were performed resorting to the commercial finite volume software package FLUENT. A well known hemodynamic phenomenon, known as Fahraeus-Lindqvist effect [1, 2], observed in both in vivo and in vitro studies, results in the formation of a marginal cell-free layer (CFL) at regions adjacent to the wall [3]. Recently, studies have shown that the formation of the CFL is affected by the geometry of the microchannel and for the case of the confluences a CFL tend to appear in the middle of the microchannel after the apex of the confluence [4, 5]. By using the CFL experimental data, the main objective of this work is to implement a CFL in the numerical simulations in order to obtain a better understanding of the effect of this layer on the velocity profiles.