Percorrer por autor "Ferreira-Santos, Pedro"
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- Chemical profile and bioactivities of extracts from edible plants readily available in PortugalPublication . Silva, Beatriz Nunes; Cadavez, Vasco; Ferreira-Santos, Pedro; Alves, Maria José; Ferreira, Isabel C.F.R.; Barros, Lillian; Teixeira, José; Gonzales-Barron, UrsulaPlant extracts have been proposed as alternative biocides and antioxidants to be included in a variety of food products. In this work, to assess the potential of rosemary, lemon balm, basil, tarragon, sage, and spearmint to be used as food additives, the chemical profiles and bioactivities of such plant extracts were studied. Furthermore, to evaluate the influence of extraction methods and solvents on the chemical characteristics and bioactivities of the plant extracts, two extraction methods (solid-liquid and Soxhlet extraction) and two solvents (water and ethanol 70% (v/v)) were tested for each plant. Groupwise summary statistics were calculated by plant, extraction method, and solvent, and linear models were built to assess the main effects of those terms and their interactions on the chemical characteristics and bioactivities of the extracts. The results revealed that all factors—type of plant, extraction method and solvent—have influence on the chemical profile and antioxidant activity of the resultant extracts. Interactions between factors were also observed. Hydroethanolic Soxhlet extracts presented the least potential as biopreservatives due to their low phenolic content and reduced antioxidant capacity. Oppositely, aqueous Soxhlet extracts and hydroethanolic solid-liquid extracts showed high contents in phenolic compounds and high antioxidant activities. In particular, the hydroethanolic solid-liquid extracts of lemon balm, spearmint, and sage presented the highest phenolic and flavonoid contents, accompanied by a high antioxidant activity, and they revealed antimicrobial activity against four pathogens (S. enterica ser. Typhimurium, E. coli, L. monocytogenes and S. aureus). These results demonstrate the potential of these natural resources to be incorporated as bioactive preservatives in foods or their packaging.
- Correction: Silva et al. Chemical Profile and Bioactivities of Extracts from Edible Plants Readily Available in Portugal. Foods 2021, 10, 673Publication . Silva, Beatriz Nunes; Cadavez, Vasco; Ferreira-Santos, Pedro; Alves, Maria José; Ferreira, Isabel C.F.R.; Barros, Lillian; Teixeira, José António; Gonzales-Barron, UrsulaThe authors found a mistake in the original paper [1]. Throughout the article, French lavender was wrongfully named rosemary. Rosemary was not used in the original study. Below are provided the full details of the changes in the Figures, Tables, and text. The authors sincerely apologise for any inconvenience caused and state that the scientific conclusions are unaffected. The original publication has been updated.
- Extraction, chemical characterization, and antioxidant activity of bioactive plant extractsPublication . Silva, Beatriz Nunes; Cadavez, Vasco; Ferreira-Santos, Pedro; Teixeira, José António; Gonzales-Barron, UrsulaNatural extracts have been proposed as preservatives to increase the safety of various food products. In this work, the phytochemical and antioxidant profiles of French lavender (Lavandula stoechas), lemon balm, basil, tarragon, salvia, and spearmint extracts were studied. The results show that hydroethanolic extracts may be more effective as biopreservatives if moderate temperatures are used in the extraction process, as they revealed higher phenolic content. More specifically, the results also show that lemon balm extracts present a great potential to be used as biopreservatives, due to their high-level phenolic and flavonoid contents and antioxidant activity.
- Extracts from red eggplant: impact of ohmic heating and different extraction solvents on the chemical profile and bioactivityPublication . Ferreira-Santos, Pedro; Duca, Anna Barbara; Genisheva, Zlatina; Silva, Beatriz Nunes; Biasio, Filomena de; Botelho, Cláudia; Rocha, Cristina M.R.; Gorgoglione, Domenico; Teixeira, José AntónioEggplants contain a multitude of biocompounds with nutritional and/or biological activities. The objective of this work was to study the nutritional, chemical and bioactive value of red eggplant from Rotonda, Italy. Ohmic heating (OH) was compared to conventional heating, as different solvents were used (water, ethanol 30, 50, and 90%and methanol) for biocompounds extraction. Extracts were evaluated for their total phenolic compounds, antioxidant and antibacterial activities, and its toxicity was assessed in cells, L929 and Caco-2. The nutritional characterization of Rotonda’s eggplant demonstrated that it is rich in carbohydrates (65%), fiber (12.5%), proteins (13%), lipids (7.6%) and minerals. Potassium is the mineral with the highest concentration in the red eggplant (27.24 mg/g). Phenolic composition of the obtained extracts was dependent on the extraction method, as well as on the solvent. The use of OH method increased the extraction of biocompounds, especially when using 50% of ethanol as solvent. The main phenolic compounds found in the extracts of this eggplant variety were ellagic acid, p-coumaricic acid, epicatechin, narginin, taxifolin and kaempferol. Antioxidant activity was positively correlated with the total amounts of phenolics. Red Eggplant extracts showed activity against Gram-negative bacteria (E. coli and S. enterica), however, they did not demonstrate activity against Gram-positive bacteria. The extracts obtained did not show cytotoxic effects in fibroblast and colorectal studied cells. Ohmic heating is a sustainable technology that increases the extraction yield of biocompounds, with reduced energy consumption and the resulting extracts show low toxicity and high biological activity.