Percorrer por autor "Cardoso, Rossana V.C."
A mostrar 1 - 10 de 36
Resultados por página
Opções de ordenação
- Antioxidant and antimicrobial influence on oyster mushrooms (Pleurotus ostreatus) from substrate supplementation of calcium silicatePublication . Cardoso, Rossana V.C.; Carocho, Márcio; Fernandes, Ângela; Pinela, José; Stojković, Dejan; Soković, Marina; Zied, Diego Cunha; Cobos, Juan Diego Valenzuela; González-Paramás, Ana María; Ferreira, Isabel C.F.R.; Barros, LillianSupplementation of the substrate of mushrooms with calcium silicate and other minerals is usually used as a preventive measure against pests and other contaminants during the production of oyster mushrooms. Little is known of the effects of this supplementation on the quality of the mushrooms produced. In the work described herein, the supplementation of oyster mushrooms was performed with 5 supplementation levels (0%, 0.5%, 1%, 2% and 4%) on mushrooms from two different locations in Brazil, the two flushes of mushrooms produced were analysed in terms of phenolic compounds, organic acids, and the antioxidant, antibacterial and antifungal activities, and finally the data was subjected to a linear discriminant analysis to understand the discrimination of the supplementation percentages. Overall, intermediate supplementation until 1% seemed to have a positive effect on the mushrooms from Mogi-das-Cruzes region, while high supplementation favoured the mushrooms from the region of Presidente Prudente. Supplementation showed positive effects on the mushrooms by increasing the production of some secondary metabolites while not showing any negative cytotoxic effects.
- Bioactivity of the vitamin D2-enriched extract from surplus mushroomsPublication . Cardoso, Rossana V.C.; Fernandes, Ângela; Abreu, Rui M.V.; Mandim, Filipa; González-Paramás, Ana María; Barros, LillianA percentage as high as 20% of surplus might be generated during mushroom production. These mushrooms have low industrial application because they are in an advanced stage of maturation, or they have deformed lids and/or stems that do not meet the specifications established by retailers, so they are considered mushrooms of low economic value. In their natural state, mushrooms present very low concentrations of vitamin D2. Nonetheless, researchers have found them to be a rich source of ergosterol (pro-vitamin D2), which can be converted into vitamin D2 by artificial UV irradiation. Thus, the irradiation of surplus mushrooms to obtain vitamin D2 is a sustainable strategy to increase vitamin D availability, and, in this work, the bioactive effects and potential toxicity of vitamin D2-rich extracts were evaluated. The surplus production from Agaricus bisporus Portobello were supplied by Ponto Agrícola, Baião, north of Portugal. Ultrasonic Assisted Extraction (UAE) was used to obtain an ergosterol-rich extract, which was subsequently irradiated with UV-C to obtain a vitamin D2-rich extract. The irradiation was performed in an ultraviolet (UV-C) radiation chamber. Vitamin D2 was analysed by high performance liquid chromatography coupled with an ultraviolet detector (HPLC-UV). To evaluate the bioactivity in cell lines, four human tumour cell lines (MCF-7 - breast adenocarcinoma, NCI-H460 - non-small cell lung cancer, AGS - gastric cancer, and CaCo-2 - colorectal adenocarcinoma and one non-tumoural cell line of bone origin (h- FOB 1.19 - human osteoblasts)) were used. Cell proliferation in the presence and absence of functional extract and pure vitamin D2 was assessed using the sulforhodamine B (SRB) assay. The sample of pure vitamin D2 tested did not reveal activity at the evaluated concentrations (GI50 values > 400 μg/mL) for all the cell lines tested (tumoural and non-tumoural). However, the vitamin D2-rich extract presented effective activity in the AGS (82 μg/mL) tumoural cell line and moderate activity in the NCI-H460 (293 μg/mL) and CaCo-2 (377 μg/mL) tumour cell lines. It is noteworthy that neither vitamin D2- rich extracts nor pure vitamin D2 presented cytotoxicity against the h-FOB 1.19 (GI50 > 400 μg/mL). These results are good indicators of the feasibility of industrial surplus mushrooms as sustainable vitamin D2 food sources, and they could be of added value to promote the agricultural sector or the food and pharmaceutical industries.
- Biochemical approaches on commercial strains of Agaricus subrufescens growing under two environmental cultivation conditionsPublication . Almeida, Daiana; Cardoso, Rossana V.C.; Pereira, Carla; Alves, Maria José; Ferreira, Isabel C.F.R.; Zied, Diego Cunha; Vieira Junior, Wagner Gonçalves; Caitano, Cinthia E.C.; Fernandes, Ângela; Barros, LillianIn the present work, the effect of the cultivation process, in the field and under a con- trolled environment, on biochemical parameters by using commercial strains of A. subrufescens were evaluated. The results obtained revealed that the strains cultivated in the field presented higher levels for most of the parameters evaluated (organic acids (20.5–48.0 g/100 g dw), tocopherols (107.0–198.6 μg/100 g dw), and phenolic acids and related compounds (245.2–359.0 μg/100 g dw and 10.6–23.7 μg/100 g dw, respectively)), except for the carbohydrates (53.4–72.6 g/100 g dw), energetic value (373–380 Kcal/100 g dw), and total free sugars (28.8–43.1 g/100 g dw), parameters in which the strains grown in a controlled environment present better results. For both cultivation systems, similar results were obtained regarding saturated, monounsaturated, and polyunsaturated fatty acids, as well as antioxidant and antimicrobial activities. These data contribute to the knowledge and highlight the characterized strains and the cultivation process, which can be used to obtain ingredients with potential applicability as a source of functional compounds.
- Bioresíduos de cogumelos como fonte de vitamina D2Publication . Cardoso, Rossana V.C.; Fernandes, Ângela; Barreira, João C.M.; González-Paramás, Ana María; Barros, Lillian; Ferreira, Isabel C.F.R.São vários os estudos que descrevem a importância nutricional dos cogumelos enquanto alimento e como fonte de compostos bioativos com propriedades terapêuticas
- Boletus edulis as a healthy and prized edible mushroom: analysis of bioactive compounds and in vitro functional propertiesPublication . Casado-Hidalgo, Gema; Cebollada, Pilar; Cano-Lou, Javier; Cardoso, Rossana V.C.; Barros, Lillian; Rodrígez-Yoldi, Maria Jesús; López, VíctorBoletus edulis is a widely consumed edible mushroom, known for its high nutritional value and bioactive compounds of interest. This study aimed to explore its potential bioactive properties by evaluating the inhibitory effects of B. edulis extract on key digestive enzymes and its antioxidant capacity through in vitro assays. For this purpose, an hydroethanolic extract of B. edulis was evaluated phytochemically and through in vitro bioassays for antioxidant activity and enzyme inhibition. The extract contained 226 f 3 mg of ergosterol/100 g, and phenolic compounds such as epigallocatechin-3-gallate (501 f 18 mg/100 g) and caffeoyl tryptophan (15 f 1 mg/100 g), consistent with fungal species of functional interest. Cytotoxicity effects of the extract were assessed in HepG2 cells, and results showed no significant effect on the loss of cell viability. Furthermore, it showed antioxidant capacity, including superoxide radical scavenging, nitric oxide and intracellular ROS production as well as a moderate inhibition of metabolic enzymes such as alpha-glucosidase (IC50 = 13 f 1 mg/mL) and lipase (IC50 = 25 f 11 mg/mL), and the pro-inflammatory enzyme 5-lipoxygenase, with an approximate IC50 value of 2.5 mg/mL. These results evidence a multi-targeted bioactive profile that could offer cumulative health benefits with regular consumption. However, further in vivo and clinical studies are necessary to confirm its potential as functional food.
- A case study on surplus mushrooms production: extraction and recovery of vitamin DPublication . Cardoso, Rossana V.C.; Fernandes, Ângela; Barreira, João C.M.; Abreu, Rui M.V.; Mandim, Filipa; González-Paramás, Ana María; Ferreira, Isabel C.F.R.; Barros, LillianThe presented case study illustrates the possibility of adding value to the biological surplus remaining from the mushroom cultivation industry. In essence, the unused mushroom parts were submitted to UV-C irradiation, with the purpose of increasing the vitamin D2 content and validat-ing its extraction. Vitamin D2 concentration in three different mushroom species (Agaricus bisporus, A. bisporus Portobello, and Pleurotus ostreatus) was obtained by high-performance liquid chromatogra-phy (HPLC), by means of an ultraviolet (UV) detector. The method was validated using an A. bisporus Portobello sample, and its reproducibility and accuracy were confirmed. Independently of the UV-C irradiation dose, the effect on vitamin D2 concentration was significant, allowing it to increase from less than 4 µg/g dry weight (dw) to more than 100 µg/g dw in all mushroom species. These results are good indicators of the feasibility of industrial surplus mushrooms as sustainable vitamin D2 food sources, besides contributing to strengthen the circularity principals associated to the mushroom production chain.
- Cereal milling by-products as sources of nutrients and antioxidant phenolic compoundsPublication . Cardoso, Rossana V.C.; Fernandes, Ângela; Pinela, José; Dias, Maria Inês; Pereira, Carla; Carocho, Márcio; Fernández Vasallo, Esteban; Ferreira, Isabel C.F.R.; Barros, LillianCereaIs are staple food crops and major sources of nutrition wor1dwide, but their processing generates a large amount of by-products. In addition to environmental and economic aspects, cereal milling by-products represent a significant loss of natural resources and nutrients when discarded as useless waste [1,2]. Thus, in order to promote the upcyc1ing of these by-products as valuable raw materiaIs, this study was carried out to provide a compositional and bioactive characterisation of wheat, maise and rye by-products current1y produced in large quantities by the cereal grain milling industry. The cereal by-products were studied for their proximate composition folIowing official methods of food analysis, and organic acids, soluble sugars, fatty acids and tocopherols were analysed by different chromatographie techniques [3]. After preparation of hydroethanolic extracts, the detected phenolic compounds were characterised by HPLC-DAD-ESIIMSnand the antioxidant activity was evaluated through in vitro assays for the ability to inhibit lipid peroxidation by monitoring the formation of thiobarbituric acid reactive substances (TBARS), and to inhibit oxidative hemolysis caused by free radicals generated in the in vitro system [3]. Regarding the compositional results, expressed in dry weight (dw), carbohydrates (56.35-78.12 g/100 g) were the major proximate constituents of the studied cereal by-products, folIowed by proteins (11.2-30.0 g/100 g). The higher energy value (432.3 kcal/l00 g) was presented by wheat germ, which also presented the highest citric acid content (0.86 g/100 g). Sucrose was the most abundant soluble sugar in alI cereal by-products, reaching 10.4 g/100 g in wheat germ, 3.84 g/100 g in maise bran-germ mixture, and approximately 2.9 g/100 g in wheat and rye bran samples. Unsaturated fatty acids predominated in alI samples, given the high contents of linoleic (53.9- 57.1 %) and oleie (13.4-29.0%) acids. Wheat germ had the highest leveis of tocopherols (22.8 mg/l00 g) and phenolic compounds (5.7 mg/g extract, with a high content of apigenin-C-pentoside-C-hexoside). In turn, while the wheat bran extract was particularly etIective in inhibiting the formation of TBARS, the rye bran extract was the on1y one capable of protecting sheep erythrocytes from oxidative haemolysis. OveralI, these results are valid arguments to support the use of cereal by-products as underexploited alternative sources of nutrients and bioactive phenolic compounds with potential health benefits for consumers.
- Chemical and Bioactive Properties of Red Rice with Potential Pharmaceutical UsePublication . Baptista, Eugénia; Liberal, Ângela; Cardoso, Rossana V.C.; Fernandes, Ângela; Dias, Maria Inês; Pires, Tânia C.S.P.; Calhelha, Ricardo C.; García, Pablo A.; Ferreira, Isabel C.F.R.; Barreira, João C.M.Red rice has been proposed as a super-food. Accordingly, the nutritional properties (AOAC), as well as its chemical composition, including sugars (HPLC-RI), organic acids (UFLC-PDA), tocopherols (HPLD-FD), and phenolic compounds (LC-DAD-ESI/MSn), together with the main bioactive properties (antioxidant, cytotoxic, antiproliferative, and antibacterial activities), were evaluated to access its nutritional benefits and health improvement potential. The most abundant macronutrients found were carbohydrates (87.2 g/100 g dw), proceeded by proteins (9.1 g/100 g dw), fat (2.6 g/100 g dw), and ash (1.1 g/100 g dw). Sucrose and raffinose were the only detected sugars, with sucrose presenting the maximum concentration (0.74 g/100 g dw). MUFAs and PUFAs were the predominant fatty acids (40.7% and 31%, respectively). Among the two detected tocopherol isoforms, γ-tocopherol (0.67 mg/100 g dw) predominated over α-tocopherol. The phenolic compounds profile, majorly composed of flavan-3-ols, should be associated with the detected bioactivities, which may provide biological benefits to human health beyond the primary nutritional effect. Overall, the bioactive potential of red rice was comprehensively accessed.
- Chemical features and bioactivities of lactuca canadensis L., an unconventional food plant from Brazilian cerradoPublication . Liberal, Ângela; Coelho, Carla T.P.; Fernandes, Ângela; Cardoso, Rossana V.C.; Dias, Maria Inês; Pinela, José; Alves, Maria José; Severino, Vanessa G.P.; Ferreira, Isabel C.F.R.; Barros, LillianThroughout the world, people’s diet is generally quite restricted regarding the variety of plants used in their daily regime. The Unconventional Food Plant (UFP) Lactuca canadensis L. is an edible species of wild lettuce sparsely described in literature and considered to be native from the eastern and central parts of North America. To valorize this species as potential alternative food, an analysis of its nutritional, chemical, and bioactive properties was performed. The results specify the occurrence of organic acids, mainly quinic acid (127.9 ± 0.6 g/kg dry weight (dw)), polyunsaturated fatty acids (65.3%), among which are linolenic acid (44.4 ± 0.4 %), and tocopherols, mostly α-tocopherol (61.2 ± 0.7 mg/kg dw). Additionally, eight phenolic compounds were also identified, among which luteolin-O-glucuronide was found in larger amounts in both infusion and hydroethanolic extracts (5.46 ± 0.09 and 4.6 ± 0.1 mg/g dw, respectively). Carbohydrates and proteins were the main macronutrients (603 ± 1 and 177.5 ± 0.3 g/kg dw, respectively), followed by ashes (166.5 ± 0.9), indicative of a great amount of minerals. Additionally, good antioxidant and antibacterial activities were detected in the analyzed extracts. In general, our results contribute to extend the range of different, unexploited, and nutritionally balanced plant foods, such as Lactuca canadensis, that can and should be included in the daily diet.
- Combined effects of irradiation and storage time on the nutritional and chemical parameters of dried Agaricus bisporus Portobello mushroom flourPublication . Cardoso, Rossana V.C.; Carocho, Márcio; Fernandes, Ângela; Barreira, João C.M.; Cabo Verde, Sandra; Santos, Pedro M.P.; Antonio, Amilcar L.; Gonzaléz-Paramás, Ana M.; Barros, Lillian; Ferreira, Isabel C.F.R.Abstract: Portobello variety of Agaricus bisporus mushrooms, appreciated for its taste, makes it desirable to be eaten fresh and also as flour in soups and gravies. Gamma and electron-beam radiation at four doses (1, 2, 5, and 10 kGy) were used to analyze its preservation effect on Portobello mushroom flour. A proximate analysis, as well as the impact on fatty acids, tocopherols, soluble sugars, organic acids, and ergosterol profiles, were performed every 3 months, during a storage period of 1 year. Gamma rays preserved mannitol (most abundant soluble sugar) over the 12 months, while electron beam radiation preserved organic acids. No significant changes were sought for any radiation type, and the slight changes extracted from the estimated marginal means reveal a tendency for irradiation as having preserving effects of nutrients and other important molecules. Thus, both irradiation types, up to 10 kGy are suitable for preservation of A. bisporus Portobello flour.