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Title: Cytisus multiflorus: source of antioxidant polyphenols
Authors: Pereira, Olívia R.
Perez, María J.
Macias, Rócio I.R.
Domingues, M.R.M.
Silva, Artur M.S.
Marín, Jose J.G.
Cardoso, Susana M.
Keywords: Cytisus multiflorus
Phenolic compounds
Antioxidant activity
Issue Date: 2012
Publisher: Instituto Politécnico de Bragança
Citation: Pereira, Olívia R.; Perez, Maria J.; Macias, Rocio I. R.; Domingues, Maria R. M.; Silva, Artur M. S.; Marin, Jose J. G.;Cardoso, Susana M. (2012) - Cytisus multiflorus: source of antioxidant polyphenols. In 11º Encontro Química dos Alimentos. Bragança
Abstract: The present study investigates the phenolic composition and the antioxidant capacity of an ethanolic extract from flowers of C. multiflorus. As determined by HPLC-DAD, ESI-MSn and NMR combined analysis, this extract was mainly composed of chrysin-7-O-β-D-glycopyranoside, and also contained considerable amounts of rutin, a dihydroxyflavone isomer of chrysin and glycosidic derivatives of luteolin and apigenin. The C. multiflorus ethanolic extract exhibited high DPPH scavenging activity and reducing power with EC50 of 13.4 ± 0.6 and 95.7 ± 4.6 µg/mL, respectively. The exposure of human hepatoblastoma HepG2 cells to the non-toxic concentrations of the extract (50 or 200 µg/mL) resulted in a decreased rate of ROS production in a concentration dependent manner. Moreover, the mixture of standards that simulated the phenolic composition of the plant afforded a protection of about 50% in intracellular ROS production. Hence, the gathered results suggest that C. multiflorus polyphenols are closely associated to its antioxidant properties.
Peer review: yes
ISBN: 978-972-745-132-6
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Appears in Collections:DTDT - Posters em Encontros Científicos Nacionais

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