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Advisor(s)
Abstract(s)
Progress in the development of confocal microscopy and the advantages of
this technique over conventional microscopy have led to a new technique known as
confocal micro-PTV. This technique combines a manual tracking method with a
spinning disk confocal microscope. By combining its spatial filtering technique with a
multipoint illumination system, this technique has the ability to obtain in-focus images
with optical thickness less than 5 mm. The present study shows the ability of our
confocal micro-PTV system to obtain detailed qualitative and quantitative information
on the blood flow behavior in both glass capillaries and polydimethylsiloxane(PDMS)
microchannels. By labeling the blood cells with a lipophilic carbocyanime derivative it
was possible to measure both translational and rotational motion occurring during
flow. Our results demonstrate the ability of our confocal micro-PTV system to obtain
both translational and rotational motion of individual RBCs flowing in concentrated
suspensions. Owing to its optical sectioning ability and consequent improvement of the
image contrast and definition, the proposed confocal system can provide additional
detailed description on the blood cells motion not obtainable by other conventional
methods.
Description
Keywords
Blood flow Confocal micro-PIV/PTV
Citation
Lima, Rui; Ishikawa, T.; Imai, Y.; Yamaguchi, T. (2010). Confocal micro-flow visualization of blood cells. In V European Conference on Computational Fluid Dynamics, ECCOMAS CFD. Lisbon, Portugal.
Publisher
J. C. F. Pereira and A. Sequeira (Eds)