Publication
Confocal micro-flow visualization of blood cells
| dc.contributor.author | Lima, Rui A. | |
| dc.contributor.author | Ishikawa, Takuji | |
| dc.contributor.author | Imai, Yohsuke | |
| dc.contributor.author | Yamaguchi, Takami | |
| dc.date.accessioned | 2011-06-03T11:37:43Z | |
| dc.date.available | 2011-06-03T11:37:43Z | |
| dc.date.issued | 2010 | |
| dc.description.abstract | Progress in the development of confocal microscopy and the advantages of this technique over conventional microscopy have led to a new technique known as confocal micro-PTV. This technique combines a manual tracking method with a spinning disk confocal microscope. By combining its spatial filtering technique with a multipoint illumination system, this technique has the ability to obtain in-focus images with optical thickness less than 5 mm. The present study shows the ability of our confocal micro-PTV system to obtain detailed qualitative and quantitative information on the blood flow behavior in both glass capillaries and polydimethylsiloxane(PDMS) microchannels. By labeling the blood cells with a lipophilic carbocyanime derivative it was possible to measure both translational and rotational motion occurring during flow. Our results demonstrate the ability of our confocal micro-PTV system to obtain both translational and rotational motion of individual RBCs flowing in concentrated suspensions. Owing to its optical sectioning ability and consequent improvement of the image contrast and definition, the proposed confocal system can provide additional detailed description on the blood cells motion not obtainable by other conventional methods. | por |
| dc.description.sponsorship | This study was supported in part by the following grants: Grant-in-Aid for Science and Technology (PTDC/SAU-BEB/108728/2008 and PTDC/SAU-BEB/105650/2008) from the Science and Technology Foundation (FCT) and COMPETE, Portugal and Grant-in- Aid for Scientific Research (S) from the Japan Society for the Promotion of Science (JSPS; No.19100008). We also acknowledge the support from the 2007 Global COE Program “Global Nano-Biomedical Engineering Education and Research Network”. | |
| dc.identifier.citation | Lima, Rui; Ishikawa, T.; Imai, Y.; Yamaguchi, T. (2010). Confocal micro-flow visualization of blood cells. In V European Conference on Computational Fluid Dynamics, ECCOMAS CFD. Lisbon, Portugal. | por |
| dc.identifier.uri | http://hdl.handle.net/10198/4983 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | J. C. F. Pereira and A. Sequeira (Eds) | por |
| dc.relation | Hemo-Networks - Experimental and computational investigations of the factors influencing blood flow in microvascular networks. | |
| dc.subject | Blood flow | por |
| dc.subject | Confocal micro-PIV/PTV | por |
| dc.title | Confocal micro-flow visualization of blood cells | por |
| dc.type | conference paper | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Hemo-Networks - Experimental and computational investigations of the factors influencing blood flow in microvascular networks. | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-BEB%2F105650%2F2008/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-BEB%2F108728%2F2008/PT | |
| oaire.citation.conferencePlace | Lisbon, Portugal | por |
| oaire.citation.title | V European Conference on Computational Fluid Dynamics, ECCOMAS CFD 2010 | por |
| oaire.fundingStream | 5876-PPCDTI | |
| oaire.fundingStream | 5876-PPCDTI | |
| person.familyName | Lima | |
| person.givenName | Rui A. | |
| person.identifier.ciencia-id | EE12-C3FB-349D | |
| person.identifier.orcid | 0000-0003-3428-637X | |
| person.identifier.rid | H-5157-2016 | |
| person.identifier.scopus-author-id | 18437397800 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | por |
| rcaap.type | conferenceObject | por |
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