Repository logo
 
Publication

Synthesis of chitosan nanoparticles (CSNP): effect of CH-CH-TPP ratio on size and stability of NPs

2024Journal article Open access
Simple item page
dc.contributor.authorDes Bouillons-Gamboa, Rosvin E.
dc.contributor.authorOca-Vásquez, Gabriela Montes de
dc.contributor.authorVega-Baudrit, Jose Roberto
dc.contributor.authorRíos Duarte, Liz Carolina
dc.contributor.authorLopretti, Mary
dc.contributor.authorRentería Urquiza, Maite
dc.contributor.authorZúñiga-Umaña, Juan Miguel
dc.contributor.authorBarreiro, M.F.
dc.contributor.authorVázquez, Patricia
dc.date.accessioned2025-01-13T10:40:09Z
dc.date.available2025-01-13T10:40:09Z
dc.date.issued2024
dc.description.abstractIn the face of a pressing global issue-the escalating threat of antibiotic resistancethe development of new antimicrobial agents is urgent. Nanotechnology, with its innovative approach, emerges as a promising solution to enhance the efficacy of these agents and combat the challenge of microbial resistance. Chitosan nanoparticles (CSNPs) stand out in biomedical applications, particularly in the controlled release of antibiotics, with their unique properties such as biocompatibility, stability, biodegradability, non-toxicity, and simple synthesis processes suitable for sensitive molecules. This study synthesized CSNPs using the ionotropic gelation method, with tripolyphosphate (TPP) as the crosslinking agent. Various CS: TPP ratios (6:1, 5:1, 4:1, 3:1, 2:1) were tested, and the resulting nanoparticles were evaluated using dynamic light scattering (DLS). The CS: TPP ratio of 4:1, with an average hydrodynamic diameter (DHP) of (195 ± 10) nm and a zeta potential of (51 ± 1) mV, was identified as the most suitable for further analysis. The characterization of NPs by Transmission Electron Microscope (TEM) and atomic force microscopy (AFM) revealed diameters of (65 ± 14) nm and (102 ± 18) nm, respectively. Notably, CSNPs exhibited significant aggregation during centrifugation and lyophilization, leading to diameter increases of up to 285% as measured by AFM. The antibacterial activity of CSNPs against Staphylococcus aureus and Escherichia coli was assessed using the resazurin assay. It was found that CSNPs not subjected to centrifugation, freezing, and lyophilization retained their antimicrobial activity. In contrast, those that underwent these processes lost their efficacy, likely due to aggregation and destabilization of the system. This study presents a straightforward and effective protocol for encapsulating sensitive active agents and synthesizing chitosan nanoparticles, a potential system with significant implications in the fight against antibiotic resistance.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBouillons-Gamboa, Rosvin E. Des; Oca-Vásquez, Gabriela Montes de; Vega-Baudrit, Jose Roberto; Ríos-Duarte, Liz Carolina; Lopretti, Mary; Rentería-Urquiza, Maite; Zúñiga-Umaña, Juan Miguel; Barreiro, Filomena; Vázquez, Patricia (2024). Synthesis of chitosan nanoparticles (CSNP): effect of CH-CH-TPP ratio on size and stability of NPs. Frontiers in Chemistry. ISSN 2296-2646. 12, p. 1-14pt_PT
dc.identifier.doi10.3389/fchem.2024.1469271pt_PT
dc.identifier.issn2296-2646
dc.identifier.urihttp://hdl.handle.net/10198/30926
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontierspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectChitosanpt_PT
dc.subjectNanoparticlespt_PT
dc.subjectSynthesispt_PT
dc.subjectAntibiotic resistancept_PT
dc.subjectAFMpt_PT
dc.subjectDLSpt_PT
dc.subjectTEMpt_PT
dc.titleSynthesis of chitosan nanoparticles (CSNP): effect of CH-CH-TPP ratio on size and stability of NPspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage14pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleFrontiers in Chemistrypt_PT
oaire.citation.volume12pt_PT
person.familyNameBarreiro
person.givenNameFilomena
person.identifier.ciencia-id3418-47D5-5746
person.identifier.orcid0000-0002-6844-333X
person.identifier.ridL-9802-2014
person.identifier.scopus-author-id57188719717
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication0391987c-ba90-488b-839a-153447ce1f0f
relation.isAuthorOfPublication.latestForDiscovery0391987c-ba90-488b-839a-153447ce1f0f

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Synthesis_of_chitosan_nanoparticles.pdf
Size:
3.28 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIMO - Artigos em Revistas Indexados à WoS/Scopus