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Advisor(s)
Abstract(s)
The extensive use of water and anthropogenic activities associated with the inappropriate
use of antibiotics and their overuse throughout history are one of the causes for the high
incidence of antimicrobial-resistant genes (ARGs) and bacteria (ARBs) isolated from
aquatic ecosystems. According to the World Health Organization (WHO) ARGs and ARBs
is considered as a major public health concern. Aeromonas spp. are ubiquitous bacteria,
primarily recovered from aquatic ecosystems. They have been isolated from wastewater,
natural water such as rivers, lakes and estuaries, aquacultures, urban drinking water, and
in association with numerous autochthonous aquatic organisms in these environments.
This study aimed to evaluate antimicrobial resistance among riverine Aeromonas spp.,
taken as representative of the autochthonous microbiota, to assess the level of
antibacterial resistance in water, and the potential risk that it represents. Water samples
were collected from the hydrographic basins of Tua river, Portugal. Samples were filtered
through a cellulose nitrate pore membrane filter. The filters were incubated at 37°C for 24
h in Glutamate Starch Phenol red (GSP) agar. Aeromonas isolates were identified by API
20 NE. Antimicrobial susceptibility testing was performed by the Kirby-Bauer disk
diffusion method. A broad range of antibiotics covering the β-lactams family were used
to determine the resistance of isolates. The results indicate a greater incidence of multiple
antibiotic resistance Aeromonas isolates, as follows: AML-amoxicillin (93.33%); AMCamoxicillin/
clavulanic acid (73.33%); TIC-ticarcillin (83.33%); TIM-ticarcillin/clavulanic
acid (56.67%); PRL-piperacillin (40.00%); TZP-piperacillin/tazobactam (40.00%); ATMaztreonam
(33.33%); IPM-imipenem (43.33%); KF-cephalothin (70.00%); CTX-cefotaxime
(40.00%). The high aeromonads β-lactamases resistance suggest that this species can
be used as bioindicator organisms for monitoring ARGs in rivers, and should be
considered in a “One Health—One World” concept
Description
Keywords
Aeromonas spp Antimicrobial resistance Anthropogenic pressures One health concept
Pedagogical Context
Citation
Piteira, Martinha; Gomes, Sónia; Matos, M.; Fernandes, Conceição; Varandas, Simone; Saavedra, Maria José (2021). Diversity of aeromonas species isolated from surface waters: occurrence of antibiotic resistance to β-lactamase. In Livro de Resumos das XIII Jornadas de Genética e Biotecnologia. Vila Real
Publisher
Universidade de Trás-os-Montes e Alto Douro