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Advisor(s)
Abstract(s)
MiChestnut3 is a project of the company DEIFIL whose main objective is to produce more resistant
and productive hybrid chestnut (Castanea sativa Mill.) seedlings through micropropagation and
mycorrhization techniques. In this work, in addition to the agronomic traits of the micropropagated
mycorrhizal chestnut seedlings, it was also important to evaluate the changes induced by
mycorrhization in the phenolic profile of these plants. Phenolic compounds are plant secondary
metabolites involved in plant-microbe interactions/symbiosis and act as signaling molecules in the
establishment of arbuscular mycorrhizal symbioses, as well as in plant defense mechanisms [1].
According to the literature, considerable increases in phenolic compounds in host plants as a result of
arbuscular mycorrhizal fungus inoculation have been reported during the progression of the infection
[1]. Therefore, this work was carried out to study the impact of the type of fungal inoculum and the
period of mycorrhization (before or after potting) on the qualitative and quantitative profile of
phenolic compounds of the roots and leaves of the chestnut seedlings produced by DEIFIL. After
collection and lyophilization of the plant material, hydroethanolic extracts were prepared and the
phenolic compounds were characterized by HPLC-DAD-ESI/MS [2]. Ellagic acid derivatives and Oglycosylated
flavonoids were the major phenolic compounds in both plant roots and leaves, which
agreed with previous reports [2,3]. A statistical analysis showed that the type of inoculum and period
of mycorrhization significantly (p<0.05) affected the phenolic profile of the chestnut hybrids. In
general, the mycorrhizal seedlings with the fungi Amanita caesarea and Boletus edulis were those that
presented the highest levels of phenolic compounds. Relationships between the levels of these
signaling compounds and the agronomic performance of chestnut seedlings were also found. The
results bring new perspectives into the future production of a hybrid chestnut tree resistant to ink
disease in the main traditional Portuguese varieties
Description
Keywords
MiChestnut3 Castanea sativa Mill. Research Subject Categories::FORESTRY, AGRICULTURAL SCIENCES and LANDSCAPE PLANNING::Product science::Food science
Pedagogical Context
Citation
Dias, Maria Inês; Pinela, José; Pereira, Carla; Ferreira, Patrícia; Oliveira, Maria de Fátima; Martins, Anabela; Afonso, Andreia; Barros, Lillian (2022). Mycorrhization and micropropation of chestnut (Castanea sativa Mill.) seedlings as tools to obtain high added-value phenolic compounds. In 1st European Symposium on Phytochemicals in Medicine and Food.1-EuSPMF: Book of abstracts. Sérvia
Publisher
University of Belgrade