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Blood flow visualization and measurements in microfluidic devices fabricated by a micromilling technique

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Abstract(s)

The most common and used technique to produce microfluidic devices for biomedical applications is the soft-lithography. However, this is a high cost and time-consuming technique. Recently, manufacturers were able to produce milling tools smaller than 100 μm and consequently have promoted the ability of the micromilling machines to fabricate microfluidic devices capable of performing cell separation. In this work, we show the ability of a micromilling machine to manufacture microchannels down to 30 μm and also the ability of a microfluidic device to perform partial separation of red blood cells from plasma. Flow visualization and measurements were performed by using a high-speed video microscopy system. Advantages and limitations of the micromilling fabrication process are also presented.

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Biomedical applications Blood cells Cell-free layer Cells separation Microfluidic devices Micromilling

Citation

Singhal, Jaron; Pinho, Diana; Lopes, Raquel; Sousa, Patricia C.; Garcia, Valdemar; Schütte, Helmut; Lima, Rui; Gassmann, Stefan (2015). Blood flow visualization and measurements in microfluidic devices fabricated by a micromilling technique. Micro and Nanosystems. ISSN 1876-4029. 7:3,p. 148-153

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