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Authentication of carnaroli rice by HRM analysis targeting nucleotide polymorphisms in the Alk and Waxy genes

2022Journal article Open access
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dc.contributor.authorGrazina, Liliana
dc.contributor.authorCosta, Joana
dc.contributor.authorAmaral, Joana S.
dc.contributor.authorGarino, Cristiano
dc.contributor.authorArlorio, Marco
dc.contributor.authorMafra, Isabel
dc.date.accessioned2022-03-14T11:30:04Z
dc.date.available2022-03-14T11:30:04Z
dc.date.issued2022
dc.description.abstractCarnaroli is a high quality and priced variety, being considered as one of the finest Italian rice varieties due to its sensorial and rheological properties and, thus being a potential adulteration target. The present work aimed at exploiting polymorphisms in the Alk (A/G and GC/TT in exon 8) and Waxy ((CT)n and G/T in intron 1) genes by HRM analysis to differentiate Carnaroli rice from closely related varieties. The HRM method targeting the Alk gene did not allow gathering the Carnaroli subgroup genotypes in the same cluster. The HRM approach targeting Waxy gene successfully discriminated the varieties sold as Carnaroli from all the others with high level of confidence (>98%), which corroborated sequencing data. Its applicability to commercial rice samples was successful. Therefore, the proposed new HRM method can be considered a simple, specific, high-throughput and cost-effective tool for the authentication of Carnaroli rice, contributing to valorise such premium variety.pt_PT
dc.description.sponsorshipThis work was funded by the European project FOODINTEGRITY (FP7-KBBE-2013-single-stage, under grant agreement No 613688), the project UIDB/50006/2020 subsidised by FCT/MCTES (Fundação para a Ciência e Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior) through national funds and the project NORTE-01-0145- FEDER-000052. L. Grazina thanks FCT and ESF (European Social Fund) through POCH (Programa Operacional Capital Humano) for her PhD grant (SFRH/BD/132462/2017). J. Costa thanks FCT for funding throughpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGrazina, Liliana; Costa, Joana; Amaral, Joana S.; Garino, Cristiano; Arlorio, Marco; Mafra, I. (2022). Authentication of carnaroli rice by HRM analysis targeting nucleotide polymorphisms in the Alk and Waxy genes. Food Control. ISSN 0956-7135. 135, p. 1-10pt_PT
dc.identifier.doi10.1016/j.foodcont.2022.108829pt_PT
dc.identifier.issn0956-7135
dc.identifier.urihttp://hdl.handle.net/10198/25214
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationEnsuring the Integrity of the European food chain
dc.relationSafety of Plant Food Supplements: advanced genomic and chemical approaches to assess botanical origin, illegal drugs and contaminants
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectOryza sativapt_PT
dc.subjectDNA markerspt_PT
dc.subjectHigh resolution melting analysispt_PT
dc.subjectVariety differentiationpt_PT
dc.subjectAuthenticitypt_PT
dc.subjectItalian rice varietiespt_PT
dc.titleAuthentication of carnaroli rice by HRM analysis targeting nucleotide polymorphisms in the Alk and Waxy genespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEnsuring the Integrity of the European food chain
oaire.awardTitleSafety of Plant Food Supplements: advanced genomic and chemical approaches to assess botanical origin, illegal drugs and contaminants
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/613688/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F132462%2F2017/PT
oaire.citation.startPage108829pt_PT
oaire.citation.titleFood Controlpt_PT
oaire.citation.volume135pt_PT
oaire.fundingStreamFP7
person.familyNameAmaral
person.givenNameJoana S.
person.identifier.ciencia-id5319-7DE8-BEDA
person.identifier.orcid0000-0002-3648-7303
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery42be2cf4-adc4-4e7f-ac60-7aab515b38cd
relation.isProjectOfPublication050e7d15-3b12-4aa1-b7a9-bd919b5696fb
relation.isProjectOfPublication087be544-6360-497c-b38f-7b723cd3a1a2
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